Literature DB >> 10702486

Diagnosis of tuberculosis based on the two specific antigens ESAT-6 and CFP10.

L A van Pinxteren1, P Ravn, E M Agger, J Pollock, P Andersen.   

Abstract

Tests based on tuberculin purified protein derivative (PPD) cannot distinguish between tuberculosis infection, Mycobacterium bovis BCG vaccination, or exposure to environmental mycobacteria. The present study investigated the diagnostic potential of two Mycobacterium tuberculosis-specific antigens (ESAT-6 and CFP10) in experimental animals as well as during natural infection in humans and cattle. Both antigens were frequently recognized in vivo and in vitro based on the induction of delayed-type hypersensitivity responses and the ability to induce gamma interferon production by lymphocytes, respectively. The combination of ESAT-6 and CFP10 was found to be highly sensitive and specific for both in vivo and in vitro diagnosis. In humans, the combination had a high sensitivity (73%) and a much higher specificity (93%) than PPD (7%).

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Year:  2000        PMID： 10702486      PMCID： PMC95842          DOI： 10.1128/CDLI.7.2.155-160.2000

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  34 in total

1.  Clinical correlates of interferon gamma production in patients with tuberculosis.

Authors:  A Sodhi; J Gong; C Silva; D Qian; P F Barnes
Journal:  Clin Infect Dis       Date:  1997-09       Impact factor: 9.079

2.  Differential T cell responses to Mycobacterium tuberculosis ESAT6 in tuberculosis patients and healthy donors.

Authors:  T Ulrichs; M E Munk; H Mollenkopf; S Behr-Perst; R Colangeli; M L Gennaro; S H Kaufmann
Journal:  Eur J Immunol       Date:  1998-12       Impact factor: 5.532

3.  Sensitivity and specificity of a gamma interferon blood test for tuberculosis infection.

Authors:  J A Streeton; N Desem; S L Jones
Journal:  Int J Tuberc Lung Dis       Date:  1998-06       Impact factor: 2.373

4.  Predominant recognition of the ESAT-6 protein in the first phase of interferon with Mycobacterium bovis in cattle.

Authors:  J M Pollock; P Andersen
Journal:  Infect Immun       Date:  1997-07       Impact factor: 3.441

5.  Comparison of antigen-specific T-cell responses of tuberculosis patients using complex or single antigens of Mycobacterium tuberculosis.

Authors:  A S Mustafa; H A Amoudy; H G Wiker; A T Abal; P Ravn; F Oftung; P Andersen
Journal:  Scand J Immunol       Date:  1998-11       Impact factor: 3.487

6.  A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10).

Authors:  Fransois-Xavier Berthet; Peter Birk Rasmussen; Ida Rosenkrands; Peter Andersen; Brigitte Gicquel
Journal:  Microbiology (Reading)       Date:  1998-11       Impact factor: 2.777

7.  Delayed-type hypersensitivity responses to ESAT-6 and MPT64 from Mycobacterium tuberculosis in the guinea pig.

Authors:  M J Elhay; T Oettinger; P Andersen
Journal:  Infect Immun       Date:  1998-07       Impact factor: 3.441

8.  Use of Mycobacterium tuberculosis complex-specific antigen cocktails for a skin test specific for tuberculosis.

Authors:  K Lyashchenko; C Manca; R Colangeli; A Heijbel; A Williams; M L Gennaro
Journal:  Infect Immun       Date:  1998-08       Impact factor: 3.441

9.  MPB64 mycobacterial antigen: a new skin-test reagent through patch method for rapid diagnosis of active tuberculosis.

Authors:  R M Nakamura; M A Velmonte; K Kawajiri; C F Ang; R A Frias; M T Mendoza; J C Montoya; I Honda; S Haga; I Toida
Journal:  Int J Tuberc Lung Dis       Date:  1998-07       Impact factor: 2.373

10.  Use of a Mycobacterium tuberculosis H37Rv bacterial artificial chromosome library for genome mapping, sequencing, and comparative genomics.

Authors:  R Brosch; S V Gordon; A Billault; T Garnier; K Eiglmeier; C Soravito; B G Barrell; S T Cole
Journal:  Infect Immun       Date:  1998-05       Impact factor: 3.441
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  110 in total

1.  Evaluation of a tuberculosis whole-blood interferon-γ chemiluminescent immunoassay among Chinese military recruits.

Authors:  Xueqiong Wu; Ying Hou; Yan Liang; Junxian Zhang; Yourong Yang; Lan Wang; Chuiying Zhang
Journal:  Mol Diagn Ther       Date:  2011-12-01       Impact factor: 4.074

2.  Identification, molecular cloning, and evaluation of potential use of isocitrate dehydrogenase II of Mycobacterium bovis BCG in serodiagnosis of tuberculosis.

Authors:  W Florio; D Bottai; G Batoni; S Esin; M Pardini; G Maisetta; M Campa
Journal:  Clin Diagn Lab Immunol       Date:  2002-07

3.  Complexes of streptavidin-fused antigens with biotinylated antibodies targeting receptors on dendritic cell surface: a novel tool for induction of specific T-cell immune responses.

Authors:  Ondrej Stanek; Irena Linhartova; Laleh Majlessi; Claude Leclerc; Peter Sebo
Journal:  Mol Biotechnol       Date:  2012-07       Impact factor: 2.695

4.  An in-house RD1-based enzyme-linked immunospot-gamma interferon assay instead of the tuberculin skin test for diagnosis of latent Mycobacterium tuberculosis infection.

Authors:  Luigi Codecasa; Paola Mantegani; Laura Galli; Adriano Lazzarin; Paolo Scarpellini; Claudio Fortis
Journal:  J Clin Microbiol       Date:  2006-06       Impact factor: 5.948

5.  Assessment of cross-reactivity between Mycobacterium bovis and M. kansasii ESAT-6 and CFP-10 at the T-cell epitope level.

Authors:  H Martin Vordermeier; Jemma Brown; Paul J Cockle; Willeke P J Franken; Jan Wouter Drijfhout; Sandra M Arend; Tom H M Ottenhoff; Keith Jahans; R Glyn Hewinson
Journal:  Clin Vaccine Immunol       Date:  2007-08-01

6.  Use of synthetic peptides derived from the antigens ESAT-6 and CFP-10 for differential diagnosis of bovine tuberculosis in cattle.

Authors:  H M Vordermeier; A Whelan; P J Cockle; L Farrant; N Palmer; R G Hewinson
Journal:  Clin Diagn Lab Immunol       Date:  2001-05

7.  The 6-kilodalton early secreted antigenic target-responsive, asymptomatic contacts of tuberculosis patients express elevated levels of interleukin-4 and reduced levels of gamma interferon.

Authors:  Abebech Demissie; Liya Wassie; Markos Abebe; Abraham Aseffa; Graham Rook; Alimuddin Zumla; Peter Andersen; T Mark Doherty
Journal:  Infect Immun       Date:  2006-05       Impact factor: 3.441

8.  Intracellular expression of Mycobacterium tuberculosis-specific 10-kDa antigen down-regulates macrophage B7.1 expression and nitric oxide release.

Authors:  B Singh; G Singh; V Trajkovic; P Sharma
Journal:  Clin Exp Immunol       Date:  2003-10       Impact factor: 4.330

9.  Specific delayed-type hypersensitivity responses to ESAT-6 identify tuberculosis-infected cattle.

Authors:  J M Pollock; J McNair; H Bassett; J P Cassidy; E Costello; H Aggerbeck; I Rosenkrands; P Andersen
Journal:  J Clin Microbiol       Date:  2003-05       Impact factor: 5.948

10.  Screening of predicted secreted antigens from Mycobacterium bovis reveals the immunodominance of the ESAT-6 protein family.

Authors:  Gareth J Jones; Stephen V Gordon; R Glyn Hewinson; H Martin Vordermeier
Journal:  Infect Immun       Date:  2010-01-19       Impact factor: 3.441
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