Temporal transcription map of the virulent Streptococcus thermophilus bacteriophage Sfi19.

A transcription map was developed for the virulent Streptococcus thermophilus phage Sfi19 on the basis of systematic Northern blot hybridizations. All deduced 5' ends were confirmed by primer extension experiments. Three classes of transcripts were detected based on the different times of appearance. Early transcripts were identified in three genome regions; middle transcripts covered cro-like, DNA replication, and transcriptional regulation genes; and late genes consisted of structural and lysis genes. Chloramphenicol treatment suppressed the translation of a putative transcriptional factor necessary for the production of late transcripts and shifted middle transcripts to early transcription times.