Literature DB >> 15274123

Purification of biotinylated proteins on streptavidin resin: a protocol for quantitative elution.

Jascha-N Rybak1, Simone B Scheurer, Dario Neri, Giuliano Elia.   

Abstract

The interaction between streptavidin and biotin is one of the most widely used tools in chemistry and biology. However, the release of biotinylated proteins from streptavidin resins remains a major problem, due to the extraordinary stability of this complex. We present a new protocol for the quantitative elution of biotinylated proteins from streptavidin Sepharose, featuring harsh elution conditions and competition with free biotin. The usefulness of the method was demonstrated by the quantitative recovery of biotinylated proteins from organ homogenates, obtained from mice perfused with a reactive ester derivative of biotin.

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Year:  2004        PMID： 15274123     DOI： 10.1002/pmic.200300780

Source DB:  PubMed          Journal:  Proteomics        ISSN： 1615-9853            Impact factor:   3.984


  39 in total

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4.  Proteome-wide discovery and characterizations of nucleotide-binding proteins with affinity-labeled chemical probes.

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Journal:  Mol Cell Proteomics       Date:  2012-10-01       Impact factor: 5.911

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Authors:  Zongyi Hu; Adam Rolt; Xin Hu; Christopher D Ma; Derek J Le; Seung Bum Park; Michael Houghton; Noel Southall; D Eric Anderson; Daniel C Talley; John R Lloyd; Juan C Marugan; T Jake Liang
Journal:  Cell Chem Biol       Date:  2020-05-07       Impact factor: 8.116

8.  Differential axial localization along the mouse brain vascular tree of luminal sodium-dependent glutamine transporters Snat1 and Snat3.

Authors:  Nadine Ruderisch; Daniela Virgintino; Victoria Makrides; François Verrey
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9.  Sites of alkylation of human Keap1 by natural chemoprevention agents.

Authors:  Yan Luo; Aimee L Eggler; Dongting Liu; Guowen Liu; Andrew D Mesecar; Richard B van Breemen
Journal:  J Am Soc Mass Spectrom       Date:  2007-10-02       Impact factor: 3.109

10.  Cyclic acetals as cleavable linkers for affinity capture.

Authors:  Siyeon Lee; Wei Wang; Younjoo Lee; Nicole S Sampson
Journal:  Org Biomol Chem       Date:  2015-08-21       Impact factor: 3.876
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