Stable and unstable mutations in the 5' non-translated regions of tomato aspermy virus RNAs 1 and 2 generated de novo from infectious cDNA clones containing a cauliflower mosaic virus 35S promoter.

Tomato aspermy virus RNAs derived from infectious cDNA clones exhibited a number of sequence alterations in the 5' non-translated region (NTR). These included a deletion of the first four residues in both RNAs 1 and 2, transversion of residue 5 from a G to a U in RNA 1, and transversion of A to C at position of 50 of RNA 1. These alterations were not stable in the infected plants while the insertion of a U residue between nucleotides 1 and 5 of RNA 1 was stable in the infected plants. Generation of these sequence alternations was not dependent upon either the host species or the concentration of the inoculum. The sequence alterations also did not occur on passage of wildtype virus. Rather, the sequence alterations related to transcription from the cauliflower mosaic virus 35S RNA promoter-driving infectious cDNAs. The alternations observed had no impact on symptoms or infectivity, but did affect the accumulation of specific viral RNAs. The data also demonstrated the existence of some plasticity in the sequence of the 5' NTR.