Literature DB >> 17770331

Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes.

R Kay, A Chan, M Daly, J McPherson.   

Abstract

A variant of the cauliflower mosaic virus 35S promoter with transcriptional activity approximately tenfold higher than that of the natural promoter was constructed by tandem duplication of 250 base pairs of upstream sequences. The duplicated region also acted as a strong enhancer of heterologous promoters, increasing the activity of an adjacent and divergently transcribed transferred DNA gene several hundredfold, and to a lesser extent, that of another transferred DNA gene from a remote downstream position. This optimized enhancer element should be very useful for obtaining high levels of expression of foreign genes in transgenic plants.

Entities:  

Year:  1987        PMID： 17770331     DOI： 10.1126/science.236.4806.1299

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  302 in total

1.  Identification of a mannitol transporter, AgMaT1, in celery phloem.

Authors:  N Noiraud; L Maurousset; R Lemoine
Journal:  Plant Cell       Date:  2001-03       Impact factor: 11.277

2.  Type II fish antifreeze protein accumulation in transgenic tobacco does not confer frost resistance.

Authors:  K D Kenward; J Brandle; J McPherson; P L Davies
Journal:  Transgenic Res       Date:  1999-04       Impact factor: 2.788

3.  Simultaneous antisense inhibition of two starch-synthase isoforms in potato tubers leads to accumulation of grossly modified amylopectin.

Authors:  J R Lloyd; V Landschütze; J Kossmann
Journal:  Biochem J       Date:  1999-03-01       Impact factor: 3.857

4.  Identification of eukaryotic peptide deformylases reveals universality of N-terminal protein processing mechanisms.

Authors:  C Giglione; A Serero; M Pierre; B Boisson; T Meinnel
Journal:  EMBO J       Date:  2000-11-01       Impact factor: 11.598

5.  The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture.

Authors:  V Hecht; J P Vielle-Calzada; M V Hartog; E D Schmidt; K Boutilier; U Grossniklaus; S C de Vries
Journal:  Plant Physiol       Date:  2001-11       Impact factor: 8.340

6.  Lignification in transgenic poplars with extremely reduced caffeic acid O-methyltransferase activity.

Authors:  L Jouanin; T Goujon; V de Nadaï; M T Martin; I Mila; C Vallet; B Pollet; A Yoshinaga; B Chabbert; M Petit-Conil; C Lapierre
Journal:  Plant Physiol       Date:  2000-08       Impact factor: 8.340

7.  Expression of the nos operon proteins from Pseudomonas stutzeri in transgenic plants to assemble nitrous oxide reductase.

Authors:  Shen Wan; Yaseen Mottiar; Amanda M Johnson; Kagami Goto; Illimar Altosaar
Journal:  Transgenic Res       Date:  2011-09-22       Impact factor: 2.788

8.  Nitrate efflux at the root plasma membrane: identification of an Arabidopsis excretion transporter.

Authors:  Cécile Segonzac; Jean-Christophe Boyer; Emilie Ipotesi; Wojciech Szponarski; Pascal Tillard; Brigitte Touraine; Nicolas Sommerer; Michel Rossignol; Rémy Gibrat
Journal:  Plant Cell       Date:  2007-11-09       Impact factor: 11.277

9.  Tobacco transgenic lines that express fenugreek galactomannan galactosyltransferase constitutively have structurally altered galactomannans in their seed endosperm cell walls.

Authors:  J S Grant Reid; Mary E Edwards; Cathryn A Dickson; Catherine Scott; Michael J Gidley
Journal:  Plant Physiol       Date:  2003-03       Impact factor: 8.340

10.  Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants.

Authors:  W Zhang; S Subbarao; P Addae; A Shen; C Armstrong; V Peschke; L Gilbertson
Journal:  Theor Appl Genet       Date:  2003-09-25       Impact factor: 5.699
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