Literature DB >> 15251205

Development of PCR assay to identify Pseudomonas fluorescens and its biotype.

Mauro Scarpellini1, Laura Franzetti, Antoniëtta Galli.   

Abstract

The paper provides a simple protocol that uses the polymerase chain reaction to amplify a specific portion of the 16S gene, allowing the recognition of Pseudomonas fluorescens from other group I Pseudomonas. The amplified DNA patterns of 16S rRNA and ITS1, from the restriction fragment length polymorphisms VspI, HaeIII and TaqI digestion, produced band patterns that distinguished the biotypes of Ps. fluorescens. In addition to distinguishing the biotypes C and 3 we used a phenotypical method for levan production.

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Year:  2004        PMID： 15251205     DOI： 10.1016/j.femsle.2004.05.043

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  14 in total

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