| Literature DB >> 15246372 |
Seung Jun Oh1, Christoph Mosdzianowski, Dae Yoon Chi, Jung Young Kim, Se Hun Kang, Jin Sook Ryu, Jeong Seok Yeo, Dae Hyuk Moon.
Abstract
We developed a new fully automated method for the synthesis of 3'-deoxy-3'-[18F]fluorothymidine ([18F]FLT), by modifying a commercial FDG synthesizer and its disposable fluid pathway. Optimal labeling condition was that 40 mg of precursor in acetonitrile (2 mL) was heated at 150 degrees C for 100 sec, followed by heating at 85 degrees C for 450 sec and hydrolysis with 1 N HCl at 105 degrees C for 300 sec. Using 3.7 GBq of [18F]F- as starting activity, [18F]FLT was obtained with a yield of 50.5 +/- 5.2% (n = 28, decay corrected) within 60.0 +/- 5.4 min including HPLC purification. With 37.0 GBq, we obtained 48.7 +/- 5.6% (n = 10). The [18F]FLT showed the good stability for 6 h. This new automated synthesis procedure combines high and reproducible yields with the benefits of a disposable cassette system. Copyright 2004 Elsevier Inc.Entities:
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Year: 2004 PMID： 15246372 DOI： 10.1016/j.nucmedbio.2004.01.008
Source DB: PubMed Journal: Nucl Med Biol ISSN： 0969-8051 Impact factor: 2.408