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Literature DB >> 15242958

Comparison of immunofluorescence, Western blotting, and cross-adsorption assays for diagnosis of African tick bite fever.

Mogens Jensenius¹, Pierre-Edouard Fournier, Sirkka Vene, Signe Holta Ringertz, Bjørn Myrvang, Didier Raoult.

Abstract

In testing paired serum samples from 40 consecutive cases of African tick bite fever, we detected diagnostic antibodies against spotted fever group rickettsiae in 45% of the patients by immunofluorescence assay (IFA) and in 100% of the patients by Western blotting (WB) (P < 0.01). A specific diagnosis of Rickettsia africae infection could be established in 15% of the patients by IFA and in 73% of the patients by a combination of WB and cross-adsorption assays (P < 0.01).

Entities: Disease Species

Mesh：

Year: 2004 PMID： 15242958 PMCID： PMC440600 DOI： 10.1128/CDLI.11.4.786-788.2004

Source DB: PubMed Journal: Clin Diagn Lab Immunol ISSN： 1071-412X

15 in total

1. Rickettsia africae, a tick-borne pathogen in travelers to sub-Saharan Africa.

Authors: D Raoult; P E Fournier; F Fenollar; M Jensenius; T Prioe; J J de Pina; G Caruso; N Jones; H Laferl; J E Rosenblatt; T J Marrie
Journal: N Engl J Med Date: 2001-05-17 Impact factor: 91.245

2. Kinetics of specific antibodies in Mediterranean spotted fever determined by western blotting and microimmunofluorescence.

Authors: L Beati; N Teysseire; D Raoult
Journal: Acta Virol Date: 1992-01 Impact factor: 1.162

3. Rickettsia africae sp. nov., the etiological agent of African tick bite fever.

Authors: P J Kelly; L Beati; P R Mason; L A Matthewman; V Roux; D Raoult
Journal: Int J Syst Bacteriol Date: 1996-04

Review 4. Laboratory diagnosis of rickettsioses: current approaches to diagnosis of old and new rickettsial diseases.

Authors: B La Scola; D Raoult
Journal: J Clin Microbiol Date: 1997-11 Impact factor: 5.948

5. Absorption of Rickettsia rickettsii antibodies by Rickettsia rickettsii antigens in four diagnostic tests.

Authors: K E Hechemy; R L Anacker; N L Carlo; J A Fox; H A Gaafar
Journal: J Clin Microbiol Date: 1983-03 Impact factor: 5.948

6. Line blot and western blot immunoassays for diagnosis of Mediterranean spotted fever.

Authors: D Raoult; G A Dasch
Journal: J Clin Microbiol Date: 1989-09 Impact factor: 5.948

7. Serological differentiation of murine typhus and epidemic typhus using cross-adsorption and Western blotting.

Authors: B La Scola; L Rydkina; J B Ndihokubwayo; S Vene; D Raoult
Journal: Clin Diagn Lab Immunol Date: 2000-07

8. An outbreak of spotted fever rickettsiosis in U.S. Army troops deployed to Botswana.

Authors: B L Smoak; J B McClain; J F Brundage; L Broadhurst; D J Kelly; G A Dasch; R N Miller
Journal: Emerg Infect Dis Date: 1996 Jul-Sep Impact factor: 6.883

9. Outbreak of Rickettsia africae infections in participants of an adventure race in South Africa.

Authors: P E Fournier; V Roux; E Caumes; M Donzel; D Raoult
Journal: Clin Infect Dis Date: 1998-08 Impact factor: 9.079

10. Comparison of Western immunoblotting and microimmunofluorescence for diagnosis of Mediterranean spotted fever.

Authors: N Teysseire; D Raoult
Journal: J Clin Microbiol Date: 1992-02 Impact factor: 5.948

9 in total

1. Q fever, spotted fever group, and typhus group rickettsioses among hospitalized febrile patients in northern Tanzania.

Authors: Malavika Prabhu; William L Nicholson; Aubree J Roche; Gilbert J Kersh; Kelly A Fitzpatrick; Lindsay D Oliver; Robert F Massung; Anne B Morrissey; John A Bartlett; Jecinta J Onyango; Venance P Maro; Grace D Kinabo; Wilbrod Saganda; John A Crump
Journal: Clin Infect Dis Date: 2011-08 Impact factor: 9.079

2. Seroepidemiologic study of human infections with spotted fever group Rickettsiae in North Carolina.

Authors: Meagan F Vaughn; Josie Delisle; Joey Johnson; Gaylen Daves; Carl Williams; Jodi Reber; Nicole L Mendell; Donald H Bouyer; William L Nicholson; Abelardo C Moncayo; Steven R Meshnick
Journal: J Clin Microbiol Date: 2014-09-03 Impact factor: 5.948

Comparison of immunofluorescence, Western blotting, and cross-adsorption assays for diagnosis of African tick bite fever.

1. Rickettsia africae, a tick-borne pathogen in travelers to sub-Saharan Africa.

2. Kinetics of specific antibodies in Mediterranean spotted fever determined by western blotting and microimmunofluorescence.

3. Rickettsia africae sp. nov., the etiological agent of African tick bite fever.

Review 4. Laboratory diagnosis of rickettsioses: current approaches to diagnosis of old and new rickettsial diseases.

5. Absorption of Rickettsia rickettsii antibodies by Rickettsia rickettsii antigens in four diagnostic tests.

6. Line blot and western blot immunoassays for diagnosis of Mediterranean spotted fever.

7. Serological differentiation of murine typhus and epidemic typhus using cross-adsorption and Western blotting.

8. An outbreak of spotted fever rickettsiosis in U.S. Army troops deployed to Botswana.

9. Outbreak of Rickettsia africae infections in participants of an adventure race in South Africa.

10. Comparison of Western immunoblotting and microimmunofluorescence for diagnosis of Mediterranean spotted fever.

1. Q fever, spotted fever group, and typhus group rickettsioses among hospitalized febrile patients in northern Tanzania.

2. Seroepidemiologic study of human infections with spotted fever group Rickettsiae in North Carolina.

3. A focus of dogs and Rickettsia massiliae-infected Rhipicephalus sanguineus in California.

4. Human Infections by Multiple Spotted Fever Group Rickettsiae in Tennessee.

5. Tick-borne rickettsioses, neglected emerging diseases in rural Senegal.

6. Rickettsia aeschlimannii infection, Algeria.

7. Multicenter GeoSentinel analysis of rickettsial diseases in international travelers, 1996-2008.

Review 8. Rickettsia africae in the West Indies.

9. Warmer weather linked to tick attack and emergence of severe rickettsioses.