AIM: To investigate the expression of cancer-testis (CT) antigens MAGE-1, SSX-1,CTp11 and HCA587 genes in hepatocellular carcinoma (HCC) and the possibility of applying these antigens as targets for specific immunotherapy for HCC. METHODS: Expression levels of MAGE-1, SSX-1, CTp11 and HCA587 mRNA were detected with reverse transcription polymerase chain reaction (RT-PCR) in HCC tissues and corresponding adjacent non-cancerous tissues from 105 HCC patients, 40 samples of cirrhosis and normal liver tissues. Genes of five samples with positive PCR results were sequenced. RESULTS: Of 105 HCC tissues, MAGE1, SSX-1,CTp11 and HCA587 mRNA expressions were detectable in 75.2%(79/105), 72.4%(76/105), 62.9%(66/105) and 56.2%(59/105) of HCC samples, respectively. About 93.3%(98/105), 72.4%(76/105), 48.6%(51/105) and 37.1%(39/105) of HCC tissues positively expressed at least one, two, three, and four members of CT antigens, respectively. Conversely, only SSX-1 could be detectable in 2.9%(3/105) of the corresponding adjacent non-HCC tissues in which no metastatic lesion was found. Of the latter 3 patients, biopsy samples far from tumor were obtained in 2 patients and RT-PCR indicated no expression of SSX-1 mRNA in these two samples. In addition, none of 40 samples of cirrhotic and normal liver tissues expressed CT antigen gene mRNA. DNA sequences confirmed that the RT-PCR products were true target cDNA. No relationship was found between expression of CT antigens and clinico pathological indicators such as age, gender, tumor size, degree of tumor differentiation, serum alpha-fetoprotein level and infection of hepatitis B virus or hepatitis C virus (P>0.05). CONCLUSION: CT antigens genes (MAGE-1, SSX-1, CTp11 and HCA587) are expressed with high percentage and specificity in HCC and their products are promising targets for antigen-specific immunotherapy of HCC. High frequent co-expression of multiple members of CT antigens in HCC provides possibility of polyvalent vaccinations for HCC.
AIM: To investigate the expression of cancer-testis (CT) antigens MAGE-1, SSX-1,CTp11 and HCA587 genes in hepatocellular carcinoma (HCC) and the possibility of applying these antigens as targets for specific immunotherapy for HCC. METHODS: Expression levels of MAGE-1, SSX-1, CTp11 and HCA587 mRNA were detected with reverse transcription polymerase chain reaction (RT-PCR) in HCC tissues and corresponding adjacent non-cancerous tissues from 105 HCC patients, 40 samples of cirrhosis and normal liver tissues. Genes of five samples with positive PCR results were sequenced. RESULTS: Of 105 HCC tissues, MAGE1, SSX-1,CTp11 and HCA587 mRNA expressions were detectable in 75.2%(79/105), 72.4%(76/105), 62.9%(66/105) and 56.2%(59/105) of HCC samples, respectively. About 93.3%(98/105), 72.4%(76/105), 48.6%(51/105) and 37.1%(39/105) of HCC tissues positively expressed at least one, two, three, and four members of CT antigens, respectively. Conversely, only SSX-1 could be detectable in 2.9%(3/105) of the corresponding adjacent non-HCC tissues in which no metastatic lesion was found. Of the latter 3 patients, biopsy samples far from tumor were obtained in 2 patients and RT-PCR indicated no expression of SSX-1 mRNA in these two samples. In addition, none of 40 samples of cirrhotic and normal liver tissues expressed CT antigen gene mRNA. DNA sequences confirmed that the RT-PCR products were true target cDNA. No relationship was found between expression of CT antigens and clinico pathological indicators such as age, gender, tumor size, degree of tumor differentiation, serum alpha-fetoprotein level and infection of hepatitis B virus or hepatitis C virus (P>0.05). CONCLUSION: CT antigens genes (MAGE-1, SSX-1, CTp11 and HCA587) are expressed with high percentage and specificity in HCC and their products are promising targets for antigen-specific immunotherapy of HCC. High frequent co-expression of multiple members of CT antigens in HCC provides possibility of polyvalent vaccinations for HCC.
Authors: M Peck-Radosavljevic; J Pidlich; M Bergmann; P Ferenci; C Seelos; M Wichlas; E Lipinski; M Gnant; A Gangl; F Mühlbacher Journal: J Hepatol Date: 1998-03 Impact factor: 25.083
Authors: H Chen; S Cai; Y Wang; H Zhao; J Peng; X Pang; J Zhu; X Cong; J Rui; X Leng; R Du; Y Wang; H Vaughan; J Cebon; A W Burgess; W Chen Journal: Chin Med J (Engl) Date: 2000-12 Impact factor: 2.628
Authors: P van der Bruggen; C Traversari; P Chomez; C Lurquin; E De Plaen; B Van den Eynde; A Knuth; T Boon Journal: Science Date: 1991-12-13 Impact factor: 47.728
Authors: Kunle Odunsi; Achim A Jungbluth; Elisabeth Stockert; Feng Qian; Sacha Gnjatic; Jonathan Tammela; Marilyn Intengan; Amy Beck; Bernadette Keitz; Darren Santiago; Barbara Williamson; Matthew J Scanlan; Gerd Ritter; Yao-Tseng Chen; Deborah Driscoll; Ashwani Sood; Shashikant Lele; Lloyd J Old Journal: Cancer Res Date: 2003-09-15 Impact factor: 12.701
Authors: E Stockert; E Jäger; Y T Chen; M J Scanlan; I Gout; J Karbach; M Arand; A Knuth; L J Old Journal: J Exp Med Date: 1998-04-20 Impact factor: 14.307
Authors: B Thurner; I Haendle; C Röder; D Dieckmann; P Keikavoussi; H Jonuleit; A Bender; C Maczek; D Schreiner; P von den Driesch; E B Bröcker; R M Steinman; A Enk; E Kämpgen; G Schuler Journal: J Exp Med Date: 1999-12-06 Impact factor: 14.307
Authors: Maurizio Chiriva-Internati; Fabio Grizzi; Cynthia A Jumper; Everardo Cobos; Paul L Hermonat; Eldo E Frezza Journal: World J Gastroenterol Date: 2005-11-14 Impact factor: 5.742
Authors: Natalay Kouprina; Vladimir N Noskov; Adam Pavlicek; N Keith Collins; Pamela D Schoppee Bortz; Chris Ottolenghi; Dmitri Loukinov; Paul Goldsmith; John I Risinger; Jung-Hyun Kim; V Anne Westbrook; Gregory Solomon; Hanna Sounders; John C Herr; Jerzy Jurka; Victor Lobanenkov; David Schlessinger; Vladimir Larionov Journal: PLoS One Date: 2007-04-04 Impact factor: 3.240
Authors: V D'Arcy; N Pore; F Docquier; Z K Abdullaev; I Chernukhin; G-X Kita; S Rai; M Smart; D Farrar; S Pack; V Lobanenkov; E Klenova Journal: Br J Cancer Date: 2008-01-15 Impact factor: 7.640