| Literature DB >> 15225715 |
Atsushi Ogawa1, Naotoshi Tomita, Naoko Kikuchi, Shinsuke Sando, Yasuhiro Aoyama.
Abstract
An affinity column immobilizing a decapeptide H(2)N-RGDSPASSKP-CO(2)H was used to select RGD-binding aptamers from a pool of 86-mer single-strand oligodeoxynucleotides (ODNs) containing a random 40-mer sequence. The enriched library thus obtained was further selected against adsorbed fibronectin and individual aptamers were monocloned in E. coli and sequenced to give a couple of highly homologous ODNs, which indeed inhibited fibronectin-integrin mediated cell adhesion.Entities:
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Year: 2004 PMID： 15225715 DOI： 10.1016/j.bmcl.2004.05.042
Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823