Literature DB >> 15220533

Functional analysis of the spindle-checkpoint proteins using an in vitro ubiquitination assay.

Zhanyun Tang1, Hongtao Yu.   

Abstract

The spindle checkpoint helps to ensure the fidelity of chromosome segregation during mitosis and meiosis. In response to sister chromatids not properly attached to the mitotic spindle, this checkpoint blocks the activity of a large ubiquitin protein ligase complex, called the anaphase-promoting complex (APC) or cyclosome. This chapter describes the detailed protocols of an in vitro ubiquitination assay reconstituted with purified APC, cofactors, other ubiquitination enzymes, and the spindle-checkpoint proteins. This assay is extremely useful in dissecting the biochemical functions of various spindle-checkpoint proteins.

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Year:  2004        PMID： 15220533     DOI： 10.1385/1-59259-811-0:227

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  21 in total

1.  Mechanistic insight into the allosteric activation of a ubiquitin-conjugating enzyme by RING-type ubiquitin ligases.

Authors:  Engin Ozkan; Hongtao Yu; Johann Deisenhofer
Journal:  Proc Natl Acad Sci U S A       Date:  2005-12-19       Impact factor: 11.205

2.  ATP binding and ATP hydrolysis play distinct roles in the function of 26S proteasome.

Authors:  Chang-Wei Liu; Xiaohua Li; David Thompson; Kerry Wooding; Tsui-ling Chang; Zhanyun Tang; Hongtao Yu; Philip J Thomas; George N DeMartino
Journal:  Mol Cell       Date:  2006-10-06       Impact factor: 17.970

3.  A role for Cdc2- and PP2A-mediated regulation of Emi2 in the maintenance of CSF arrest.

Authors:  Qiju Wu; Yanxiang Guo; Ayumi Yamada; Jennifer A Perry; Michael Z Wang; Marito Araki; Christopher D Freel; Jeffrey J Tung; Wanli Tang; Seth S Margolis; Peter K Jackson; Hiroyuki Yamano; Maki Asano; Sally Kornbluth
Journal:  Curr Biol       Date:  2007-02-06       Impact factor: 10.834

4.  Structural analysis of human Cdc20 supports multisite degron recognition by APC/C.

Authors:  Wei Tian; Bing Li; Ross Warrington; Diana R Tomchick; Hongtao Yu; Xuelian Luo
Journal:  Proc Natl Acad Sci U S A       Date:  2012-10-22       Impact factor: 11.205

5.  Bimodal activation of BubR1 by Bub3 sustains mitotic checkpoint signaling.

Authors:  Joo Seok Han; Benjamin Vitre; Daniele Fachinetti; Don W Cleveland
Journal:  Proc Natl Acad Sci U S A       Date:  2014-09-22       Impact factor: 11.205

6.  O-GlcNAcylation Antagonizes Phosphorylation of CDH1 (CDC20 Homologue 1).

Authors:  Jie Tian; Qizhi Geng; Yuehe Ding; Ji Liao; Meng-Qiu Dong; Xingzhi Xu; Jing Li
Journal:  J Biol Chem       Date:  2016-04-14       Impact factor: 5.157

7.  Catalytic assembly of the mitotic checkpoint inhibitor BubR1-Cdc20 by a Mad2-induced functional switch in Cdc20.

Authors:  Joo Seok Han; Andrew J Holland; Daniele Fachinetti; Anita Kulukian; Bulent Cetin; Don W Cleveland
Journal:  Mol Cell       Date:  2013-06-20       Impact factor: 17.970

8.  BubR1 blocks substrate recruitment to the APC/C in a KEN-box-dependent manner.

Authors:  Pablo Lara-Gonzalez; Maria I F Scott; Maria Diez; Onur Sen; Stephen S Taylor
Journal:  J Cell Sci       Date:  2011-12-22       Impact factor: 5.285

9.  A novel motif governs APC-dependent degradation of Drosophila ORC1 in vivo.

Authors:  Marito Araki; Hongtao Yu; Maki Asano
Journal:  Genes Dev       Date:  2005-09-29       Impact factor: 11.361

10.  Multiple anaphase-promoting complex/cyclosome degrons mediate the degradation of human Sgo1.

Authors:  Zemfira Karamysheva; Laura A Diaz-Martinez; Sara E Crow; Bing Li; Hongtao Yu
Journal:  J Biol Chem       Date:  2008-11-17       Impact factor: 5.157
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