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Literature DB >> 15157893

Analysis of chromatin in fission yeast.

Alison Pidoux¹, Barbara Mellone, Robin Allshire.

Abstract

The use of fission yeast as a model system for studies of chromosome biology has contributed to several key advances in the last few years. The structure of its large complex centromeres and composition of its transcriptionally silent heterochromatin resemble those of metazoa. The application of chromatin immunoprecipitation to fission yeast has been instrumental in these advances and we describe an improved version of this technique in detail. In addition, we describe several other techniques, which are useful in the analysis of chromatin in fission yeast.

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Year: 2004 PMID： 15157893 DOI： 10.1016/j.ymeth.2003.11.021

Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608

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Cited

36 in total

1. The fission yeast inhibitor of growth (ING) protein Png1p functions in response to DNA damage.

Authors: Jian-Qiang Chen; Yang Li; Xian Pan; Bing-Kun Lei; Cheng Chang; Zheng-Xun Liu; Hong Lu
Journal: J Biol Chem Date: 2010-03-18 Impact factor: 5.157

2. BAF53/Arp4 homolog Alp5 in fission yeast is required for histone H4 acetylation, kinetochore-spindle attachment, and gene silencing at centromere.

Authors: Aki Minoda; Shigeaki Saitoh; Kohta Takahashi; Takashi Toda
Journal: Mol Biol Cell Date: 2004-10-13 Impact factor: 4.138

Analysis of chromatin in fission yeast.

1. The fission yeast inhibitor of growth (ING) protein Png1p functions in response to DNA damage.

2. BAF53/Arp4 homolog Alp5 in fission yeast is required for histone H4 acetylation, kinetochore-spindle attachment, and gene silencing at centromere.

3. A Rik1-associated, cullin-dependent E3 ubiquitin ligase is essential for heterochromatin formation.

Review 4. Histone H3 variants and their potential role in indexing mammalian genomes: the "H3 barcode hypothesis".

5. Fission yeast Cid12 has dual functions in chromosome segregation and checkpoint control.

Review 6. RNA-induced initiation of transcriptional silencing (RITS) complex structure and function.

7. Stc1: a critical link between RNAi and chromatin modification required for heterochromatin integrity.

8. The fission yeast homologue of CENP-B, Abp1, regulates directionality of mating-type switching.

9. Structural diversity and dynamics of genomic replication origins in Schizosaccharomyces pombe.

10. The impact of local genome sequence on defining heterochromatin domains.