Literature DB >> 15157885

Choosing and using Schizosaccharomyces pombe plasmids.

Rania Siam1, William P Dolan, Susan L Forsburg.   

Abstract

A wide range of plasmids has been developed for molecular studies in the fission yeast Schizosaccharomyces pombe. This includes general purpose episomes, expression vectors, epitope tagging plasmids, and integration vectors. This review describes the typical features of S. pombe vectors, including replication origins, positive and negative selection markers, and constitutive and inducible promoter systems. We will also discuss vectors with epitope tags and how these can be used to modify episomal or endogenous gene sequences. Considerations for choosing and using a plasmid are presented and specialized methods are described.

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Year:  2004        PMID： 15157885     DOI： 10.1016/j.ymeth.2003.11.013

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  24 in total

1.  Mfc1 is a novel forespore membrane copper transporter in meiotic and sporulating cells.

Authors:  Jude Beaudoin; Raphaël Ioannoni; Luis López-Maury; Jürg Bähler; Samia Ait-Mohand; Brigitte Guérin; Sheel C Dodani; Christopher J Chang; Simon Labbé
Journal:  J Biol Chem       Date:  2011-08-02       Impact factor: 5.157

2.  Organellar mechanosensitive channels in fission yeast regulate the hypo-osmotic shock response.

Authors:  Yoshitaka Nakayama; Kenjiro Yoshimura; Hidetoshi Iida
Journal:  Nat Commun       Date:  2012       Impact factor: 14.919

3.  RING domain dimerization is essential for RNF4 function.

Authors:  Chu Wai Liew; Huaiyu Sun; Tony Hunter; Catherine L Day
Journal:  Biochem J       Date:  2010-10-01       Impact factor: 3.857

4.  Ppc89 links multiple proteins, including the septation initiation network, to the core of the fission yeast spindle-pole body.

Authors:  Joshua A Rosenberg; Gregory C Tomlin; W Hayes McDonald; Brian E Snydsman; Eric G Muller; John R Yates; Kathleen L Gould
Journal:  Mol Biol Cell       Date:  2006-06-14       Impact factor: 4.138

5.  S. pombe replication protein Cdc18 (Cdc6) interacts with Swi6 (HP1) heterochromatin protein: region specific effects and replication timing in the centromere.

Authors:  Pao-Chen Li; Louise Chretien; Jocelyn Côté; Thomas J Kelly; Susan L Forsburg
Journal:  Cell Cycle       Date:  2011-01-15       Impact factor: 4.534

6.  The S. pombe adaptor protein Bbc1 regulates localization of Wsp1 and Vrp1 during endocytic actin patch assembly.

Authors:  Cameron Dale MacQuarrie; MariaSanta C Mangione; Robert Carroll; Michael James; Kathleen L Gould; Vladimir Sirotkin
Journal:  J Cell Sci       Date:  2019-09-11       Impact factor: 5.285

7.  Conserved function of RNF4 family proteins in eukaryotes: targeting a ubiquitin ligase to SUMOylated proteins.

Authors:  Huaiyu Sun; Joel D Leverson; Tony Hunter
Journal:  EMBO J       Date:  2007-08-30       Impact factor: 11.598

8.  Human myosin 1e tail but not motor domain replaces fission yeast Myo1 domains to support myosin-I function during endocytosis.

Authors:  Sarah R Barger; Michael L James; Christopher D Pellenz; Mira Krendel; Vladimir Sirotkin
Journal:  Exp Cell Res       Date:  2019-09-19       Impact factor: 3.905

9.  Low-copy episomal vector pFY20 and high-saturation coverage genomic libraries for the fission yeast Schizosaccharomyces pombe.

Authors:  Wayne P Wahls; Mari K Davidson
Journal:  Yeast       Date:  2008-09       Impact factor: 3.239

10.  The impact of local genome sequence on defining heterochromatin domains.

Authors:  Bayly S Wheeler; Jared A Blau; Huntington F Willard; Kristin C Scott
Journal:  PLoS Genet       Date:  2009-04-10       Impact factor: 5.917
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