| Literature DB >> 15153623 |
Claire Whitton1, Jennifer Daub, Marian Thompson, Mark Blaxter.
Abstract
Generating expressed sequence tags is a simple, cheap, and efficient way to sample the genome of a target organism. An expressed sequence tag (EST) is a single-pass sequence derived from a single complementary DNA (cDNA) clone, and the sequence serves to identify the gene from which it derives. We present a set of tested laboratory protocols for setting up and performing an EST analysis of any chosen species. These medium-throughput protocols do not require dedicated genomics equipment, such as robots, and focus on the use of microtiter plates and multichannels. Using these protocols, a single competent research worker should be able to generate 2000 ESTs in 1 mo. In a nonnormalized library, these 2000 ESTs should identify between 1000 and 1500 different genes, and thus possibly between 10 and 20% of the genes of any target parasite.Mesh：
Substances：
Year: 2004 PMID： 15153623 DOI： 10.1385/1-59259-793-9:075
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745