| Literature DB >> 15136736 |
Nathalie Beaujean1, Jane E Taylor, Michelle McGarry, John O Gardner, Ian Wilmut, Pasqualino Loi, Grazyna Ptak, Cesare Galli, Giovanna Lazzari, Adrian Bird, Lorraine E Young, Richard R Meehan.
Abstract
In contrast to mice, in sheep no genome-wide demethylation of the paternal genome occurs within the first postfertilization cell cycle. This difference could be due either to an absence of a sheep demethylase activity that is present in mouse ooplasm or to an increased protection of methylated cytosine residues in sheep sperm. Here, we use interspecies intracytoplasmic sperm injection to demonstrate that sheep sperm DNA can be demethylated in mouse oocytes. Surprisingly, mouse sperm can also be demethylated to a limited extent in sheep oocytes. Our results suggest that the murine demethylation process is facilitated either by a sperm-derived factor or by male pronuclear chromatin composition.Entities:
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Year: 2004 PMID： 15136736 PMCID： PMC419658 DOI： 10.1073/pnas.0400730101
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205