AIM: The aim of this study was to describe an auxiliary combined liver-small bowel transplantation model with the preservation of duodenum, head of pancreas and hepatic biliary system in pigs. The technique, feasibility, security and immunosuppression were commented. METHODS: Forty outbred long-white pigs were randomized into two groups, and the auxiliary composite liver/small bowel allotransplantations were undertaken in 10 long-white pigs in each group with the recipient liver preserved. Group A was not treated with immunosuppressive drugs while group B was treated with cyclosporine A and methylprednisolone after operation. The hemodynamic changes and amylase of body fluid (including blood, urine and abdominal drain) were analyzed. RESULTS: The average survival time of the animals was 10+/-1.929 d (6 to 25 d) in group A while more than 30 d in group B. The pigs could tolerate the hemodynamic fluctuation during operation and the hemodynamic parameters recovered to normal 2 h after blood reperfusion. The transient high amylase level was decreased to normal one week after operation and autopsy showed no pancreatitis. CONCLUSION: Auxiliary en-bloc liver-small bowel transplantation with partial pancreas preservation is a feasible and safe model with simplified surgical techniques for composite liver/small bowel transplantation. This model may be used as a preclinical training model for clinical transplantation method, clinical liver-small bowel transplantation related complication research, basic research including immunosuppressive treatment, organ preservation, acute rejection, chronic rejection, immuno-tolerance and xenotransplantation.
AIM: The aim of this study was to describe an auxiliary combined liver-small bowel transplantation model with the preservation of duodenum, head of pancreas and hepatic biliary system in pigs. The technique, feasibility, security and immunosuppression were commented. METHODS: Forty outbred long-white pigs were randomized into two groups, and the auxiliary composite liver/small bowel allotransplantations were undertaken in 10 long-white pigs in each group with the recipient liver preserved. Group A was not treated with immunosuppressive drugs while group B was treated with cyclosporine A and methylprednisolone after operation. The hemodynamic changes and amylase of body fluid (including blood, urine and abdominal drain) were analyzed. RESULTS: The average survival time of the animals was 10+/-1.929 d (6 to 25 d) in group A while more than 30 d in group B. The pigs could tolerate the hemodynamic fluctuation during operation and the hemodynamic parameters recovered to normal 2 h after blood reperfusion. The transient high amylase level was decreased to normal one week after operation and autopsy showed no pancreatitis. CONCLUSION: Auxiliary en-bloc liver-small bowel transplantation with partial pancreas preservation is a feasible and safe model with simplified surgical techniques for composite liver/small bowel transplantation. This model may be used as a preclinical training model for clinical transplantation method, clinical liver-small bowel transplantation related complication research, basic research including immunosuppressive treatment, organ preservation, acute rejection, chronic rejection, immuno-tolerance and xenotransplantation.
Authors: K Abu-Elmagd; J Fung; J Bueno; D Martin; J R Madariaga; G Mazariegos; G Bond; E Molmenti; R J Corry; T E Starzl; J Reyes Journal: Ann Surg Date: 2000-11 Impact factor: 12.969
Authors: J de Ville de Goyet; A Mitchell; A D Mayer; S V Beath; P J McKiernan; D A Kelly; D Mirza; J A Buckles Journal: Transplantation Date: 2000-02-27 Impact factor: 4.939
Authors: O Goulet; D Jan; S Sarnacki; N Brousse; V Colomb; R Salomon; B Cuenod; H Piloquet; C Ricour; Y Revillon Journal: Transplant Proc Date: 1996-10 Impact factor: 1.066
Authors: L Wennberg; C G Groth; A Tibell; S Zhu; J Liu; E Rafael; J Söderlund; P Biberfeld; R E Morris; A Karlsson-Parra; O Korsgren Journal: Transplant Proc Date: 1997-08 Impact factor: 1.066
Authors: T E Starzl; T R Hakala; B W Shaw; R L Hardesty; T J Rosenthal; B P Griffith; S Iwatsuki; H T Bahnson Journal: Surg Gynecol Obstet Date: 1984-03
Authors: K R McCurry; W Parker; A H Cotterell; B C Weidner; S S Lin; L J Daniels; Z E Holzknecht; G W Byrne; L E Diamond; J S Logan; J L Platt Journal: Hum Immunol Date: 1997-12 Impact factor: 2.850