Literature DB >> 15131219

Use of the omp50 gene for identification of Campylobacter species by PCR.

Luc Dedieu1, Jean-Marie Pagès, Jean-Michel Bolla.   

Abstract

We studied the prevalence of the omp50 gene and the Omp50 protein in Campylobacter strains. Immunodetection assays and DNA-DNA hybridizations showed that most C. coli strains tested were negative and most C. jejuni and C. lari strains tested were positive. A PCR assay was developed, using the omp50 gene as a species-specific target. We propose a combination of a hippurate test and an omp50 assay to perform identification of Campylobacter species.

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Year:  2004        PMID: 15131219      PMCID: PMC404618          DOI: 10.1128/JCM.42.5.2301-2305.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  14 in total

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Authors:  J Parkhill; B W Wren; K Mungall; J M Ketley; C Churcher; D Basham; T Chillingworth; R M Davies; T Feltwell; S Holroyd; K Jagels; A V Karlyshev; S Moule; M J Pallen; C W Penn; M A Quail; M A Rajandream; K M Rutherford; A H van Vliet; S Whitehead; B G Barrell
Journal:  Nature       Date:  2000-02-10       Impact factor: 49.962

3.  Purification, characterization and sequence analysis of Omp50,a new porin isolated from Campylobacter jejuni.

Authors:  J M Bolla; E Dé; A Dorez; J M Pagès
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4.  Regulation of outer membrane porin protein synthesis in Escherichia coli K-12: ompF regulates the expression of ompC.

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Authors:  M Denis; C Soumet; K Rivoal; G Ermel; D Blivet; G Salvat; P Colin
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6.  Classification of Brucella spp. isolated from marine mammals by DNA polymorphism at the omp2 locus.

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Review 7.  Regulation by nutrient limitation.

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8.  Environmental regulation of Campylobacter jejuni major outer membrane protein porin expression in Escherichia coli monitored by using green fluorescent protein.

Authors:  Luc Dedieu; Jean-Marie Pagès; Jean-Michel Bolla
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9.  Comparison of four hippurate hydrolysis methods for identification of thermophilic Campylobacter spp.

Authors:  G K Morris; M R el Sherbeeny; C M Patton; H Kodaka; G L Lombard; P Edmonds; D G Hollis; D J Brenner
Journal:  J Clin Microbiol       Date:  1985-11       Impact factor: 5.948

10.  A point-source outbreak of campylobacteriosis associated with consumption of raw milk.

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3.  Antibacterial Mode of Action of the Daucus carota Essential Oil Active Compounds against Campylobacter jejuni and Efflux-Mediated Drug Resistance in Gram-Negative Bacteria.

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4.  Adaptive mechanisms of Campylobacter jejuni to erythromycin treatment.

Authors:  Qingqing Xia; Wayne T Muraoka; Zhangqi Shen; Orhan Sahin; Hongning Wang; Zuowei Wu; Peng Liu; Qijing Zhang
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  4 in total

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