The proteome expression patterns in adult Ascaris suum under exposure to aerobic/anaerobic environments analyzed by two-dimensional electrophoresis.

We investigated the protein expression patterns (the proteome) in adult Ascaris suum under exposure to aerobic and anaerobic environments in vitro using two-dimensional polyacrylamide gel electrophoresis. Adult A. suum were cultured in RPMI-1640 in the absence of oxygen (anaerobic) or in its presence (aerobic), and whole worm extracts and their excretory-secretory (ES) products were prepared for protein analysis. More than 200 protein spots resolved in two-dimensional gels. A comparison of aerobic versus anaerobic cultures revealed two aerobic and three anaerobic specific spots differentially expressed in adult worm extracts. ES products released during aerobic/anaerobic cultures also exhibited one aerobic and seven anaerobic specific spots in two-dimensional maps. One of the anaerobic specific protein spot was analyzed for its internal amino acid sequence (1-LLAFELAPHGIR-12; 1-VNTVNPGAVD-10) and the protein was predicted to be a putative dehydrogenase. These findings suggest that the availability of oxygen or its absence in the environment may influence protein expression patterns in parasitic nematodes.