Literature DB >> 1510661

Construction of a tRNA-embedded-ribozyme trimming plasmid.

N Yuyama1, J Ohkawa, Y Inokuchi, M Shirai, A Sato, S Nishikawa, K Taira.   

Abstract

We have combined Cotten and Birnstiel's tRNA-embedded ribozymes and our 5'- and 3'-trimming system. Although the activity of the tRNA-embedded ribozyme was ca. 30% lower than those of naked ribozymes, since the stability of the former in bovine serum was higher than those of naked ribozymes, the tRNA-embedded ribozymes appear useful especially when the 5'- and 3'-trimming units are concatenated in tandem.

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Year:  1992        PMID： 1510661     DOI： 10.1016/s0006-291x(05)81543-8

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  6 in total

Review 1.  Ribozymes: biology, biochemistry, and implications for clinical medicine.

Authors:  M Kiehntopf; E L Esquivel; M A Brach; F Herrmann
Journal:  J Mol Med (Berl)       Date:  1995-02       Impact factor: 4.599

2.  Importance of independence in ribozyme reactions: kinetic behavior of trimmed and of simply connected multiple ribozymes with potential activity against human immunodeficiency virus.

Authors:  J Ohkawa; N Yuyama; Y Takebe; S Nishikawa; K Taira
Journal:  Proc Natl Acad Sci U S A       Date:  1993-12-01       Impact factor: 11.205

3.  A multifunctional expression vector for an anti-HIV-1 ribozyme that produces a 5'- and 3'-trimmed trans-acting ribozyme, targeted against HIV-1 RNA, and cis-acting ribozymes that are designed to bind to and thereby sequester trans-activator proteins such as Tat and Rev.

Authors:  N Yuyama; J Ohkawa; T Koguma; M Shirai; K Taira
Journal:  Nucleic Acids Res       Date:  1994-11-25       Impact factor: 16.971

4.  Activation of HIV-specific ribozyme activity by self-cleavage.

Authors:  M Ventura; P Wang; T Ragot; M Perricaudet; S Saragosti
Journal:  Nucleic Acids Res       Date:  1993-07-11       Impact factor: 16.971

5.  Cleavage of full-length beta APP mRNA by hammerhead ribozymes.

Authors:  R B Denman
Journal:  Nucleic Acids Res       Date:  1993-08-25       Impact factor: 16.971

Review 6.  Ribozyme-mediated inhibition of 801-bp deletion-mutant epidermal growth factor receptor mRNA expression in glioblastoma multiforme.

Authors:  Georg Karpel-Massler; Christian Rainer Wirtz; Marc-Eric Halatsch
Journal:  Molecules       Date:  2010-06-30       Impact factor: 4.411
  6 in total

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