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Literature DB >> 15102064

Evaluation of spectral imaging for plant cell analysis.

Abstract

Fluorescence imaging at high spectral resolution is now a practical reality and has great promise in plant cell biology. Emission spectral curve data can be used computationally to distinguish spectrally similar fluorophores, or to remove autofluorescence, and to spectrally analyse autofluorescent molecules, which are especially abundant in plant tissues. Examples of these applications in plant cells are given, and a comparison is made between the current offerings in spectral imaging laser scanning confocal microscopes.

Mesh：

Substances：
Fluorescent Dyes

Year: 2004 PMID： 15102064 DOI： 10.1111/j.0022-2720.2004.01347.x

Source DB: PubMed Journal: J Microsc ISSN： 0022-2720 Impact factor: 1.758

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Cited

14 in total

1. pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants.

Authors: Tzvi Tzfira; Guo-Wei Tian; Benoît Lacroix; Shachi Vyas; Jianxiong Li; Yael Leitner-Dagan; Alexander Krichevsky; Tamir Taylor; Alexander Vainstein; Vitaly Citovsky
Journal: Plant Mol Biol Date: 2005-03 Impact factor: 4.076

2. Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities.

Authors: T D Ainsworth; M Fine; L L Blackall; O Hoegh-Guldberg
Journal: Appl Environ Microbiol Date: 2006-04 Impact factor: 4.792

3. Non-de-scanned parallel recording two-photon hyperspectral microscopy with high spectral and spatial resolution.

Authors: Szu-Yu Chen; Chiao-Sheng Lu; Chia-Hua Yeh
Journal: Biomed Opt Express Date: 2014-01-02 Impact factor: 3.732

4. Two-photon imaging with longer wavelength excitation in intact Arabidopsis tissues.

Authors: Yoko Mizuta; Daisuke Kurihara; Tetsuya Higashiyama
Journal: Protoplasma Date: 2015-01-15 Impact factor: 3.356

5. Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.

Authors: Raja Chouket; Ruikang Zhang; Agnès Pellissier-Tanon; Annie Lemarchand; Agathe Espagne; Thomas Le Saux; Ludovic Jullien
Journal: Methods Mol Biol Date: 2021

Evaluation of spectral imaging for plant cell analysis.

1. pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants.

2. Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities.

3. Non-de-scanned parallel recording two-photon hyperspectral microscopy with high spectral and spatial resolution.

4. Two-photon imaging with longer wavelength excitation in intact Arabidopsis tissues.

5. Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.

6. Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy.

7. Evaluation and optimization of multiple fluorophore analysis of a Pseudomonas aeruginosa biofilm.

8. Emission spectra profiling of fluorescent proteins in living plant cells.

9. An approach for characterizing and comparing hyperspectral microscopy systems.

10. The C-terminal propeptide of a plant defensin confers cytoprotective and subcellular targeting functions.