Literature DB >> 15100812

Bacterial chromosome extraction and isolation.

Christelle Prinz1, Jonas O Tegenfeldt, Robert H Austin, Edward C Cox, James C Sturm.   

Abstract

We have used diffusive mixing and dielectrophoretic trapping to lyse Escherichia coli cells in a microfabricated environment and trap the E. coli chromosome. We characterize the conditions needed for efficient lysis of the cells, and conditions needed for the dielectrophoretic trapping of the chromatin without the trapping of cytoplasmic proteins.

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Year:  2002        PMID： 15100812     DOI： 10.1039/b208010a

Source DB:  PubMed          Journal:  Lab Chip        ISSN： 1473-0189            Impact factor:   6.799


  11 in total

Review 1.  Sample pretreatment and nucleic acid-based detection for fast diagnosis utilizing microfluidic systems.

Authors:  Jung-Hao Wang; Chih-Hung Wang; Gwo-Bin Lee
Journal:  Ann Biomed Eng       Date:  2011-12-07       Impact factor: 3.934

2.  Integrated electrical concentration and lysis of cells in a microfluidic chip.

Authors:  Christopher Church; Junjie Zhu; Guohui Huang; Tzuen-Rong Tzeng; Xiangchun Xuan
Journal:  Biomicrofluidics       Date:  2010-10-01       Impact factor: 2.800

Review 3.  Microfluidic single-cell analysis of intracellular compounds.

Authors:  Tzu-Chiao Chao; Alexandra Ros
Journal:  J R Soc Interface       Date:  2008-10-06       Impact factor: 4.118

4.  Microfluidic long DNA sample preparation from cells.

Authors:  Paridhi Agrawal; Kevin D Dorfman
Journal:  Lab Chip       Date:  2019-01-15       Impact factor: 6.799

Review 5.  Ratchets in hydrodynamic flow: more than waterwheels.

Authors:  James C Sturm; Edward C Cox; Brandon Comella; Robert H Austin
Journal:  Interface Focus       Date:  2014-12-06       Impact factor: 3.906

Review 6.  Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.

Authors:  Kevin D Dorfman; Scott B King; Daniel W Olson; Joel D P Thomas; Douglas R Tree
Journal:  Chem Rev       Date:  2012-11-12       Impact factor: 60.622

7.  Physical manipulation of the Escherichia coli chromosome reveals its soft nature.

Authors:  James Pelletier; Ken Halvorsen; Bae-Yeun Ha; Raffaella Paparcone; Steven J Sandler; Conrad L Woldringh; Wesley P Wong; Suckjoon Jun
Journal:  Proc Natl Acad Sci U S A       Date:  2012-09-14       Impact factor: 11.205

8.  Review article-dielectrophoresis: status of the theory, technology, and applications.

Authors:  Ronald Pethig
Journal:  Biomicrofluidics       Date:  2010-06-29       Impact factor: 2.800

9.  Automated sample preparation in a microfluidic culture device for cellular metabolomics.

Authors:  Laura A Filla; Katherine L Sanders; Robert T Filla; James L Edwards
Journal:  Analyst       Date:  2016-04-27       Impact factor: 4.616

10.  DNA catenation maintains structure of human metaphase chromosomes.

Authors:  David L V Bauer; Rodolphe Marie; Kristian H Rasmussen; Anders Kristensen; Kalim U Mir
Journal:  Nucleic Acids Res       Date:  2012-10-12       Impact factor: 16.971
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