Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A new method for determining the local environment and orientation of individual side chains of membrane-binding peptides.

Literature DB >> 15099085

A new method for determining the local environment and orientation of individual side chains of membrane-binding peptides.

Matthew J Tucker¹, Zelleka Getahun, Vikas Nanda, William F DeGrado, Feng Gai.

Abstract

We studied here the binding of the mastoparan X peptide to a zwitterionic lipid bilayer (POPC) and demonstrated that nitrile-derivatized amino acids can be used to determine the hydration state (or change in hydration state) of specific sites of membrane-interactive peptides (upon binding). We have also shown that polarized ATR-FTIR measurements can further be used to uncover information regarding the spatial orientation of individual side chains as well as their conformational preference within the lipid bilayer.

Entities: Chemical

Mesh：

Substances：

Year: 2004 PMID： 15099085 DOI： 10.1021/ja032015d

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

Keyword Cloud
Cited

36 in total

1. Selective incorporation of nitrile-based infrared probes into proteins via cysteine alkylation.

Authors: Hyunil Jo; Robert M Culik; Ivan V Korendovych; William F Degrado; Feng Gai
Journal: Biochemistry Date: 2010-11-17 Impact factor: 3.162

2. Investigation of an unnatural amino acid for use as a resonance Raman probe: Detection limits, solvent and temperature dependence of the νC≡N band of 4-cyanophenylalanine.

Authors: Colin L Weeks; Alexei Polishchuk; Zelleka Getahun; William F Degrado; Thomas G Spiro
Journal: J Raman Spectrosc Date: 2008-11 Impact factor: 3.133

3. Site-Specific Spectroscopic Reporters of the Local Electric Field, Hydration, Structure, and Dynamics of Biomolecules.

Authors: Matthias M Waegele; Robert M Culik; Feng Gai
Journal: J Phys Chem Lett Date: 2011-09-23 Impact factor: 6.475

4. Simulation protocols for coherent femtosecond vibrational spectra of peptides.

Authors: Wei Zhuang; Darius Abramavicius; Tomoyuki Hayashi; Shaul Mukamel
Journal: J Phys Chem B Date: 2006-02-23 Impact factor: 2.991

5. Computational design and experimental characterization of peptides intended for pH-dependent membrane insertion and pore formation.

Authors: Yao Zhang; René Bartz; Gevorg Grigoryan; Michael Bryant; Jeff Aaronson; Stephen Beck; Nathalie Innocent; Lee Klein; William Procopio; Tom Tucker; Vasant Jadhav; David M Tellers; William F DeGrado
Journal: ACS Chem Biol Date: 2015-01-28 Impact factor: 5.100

A new method for determining the local environment and orientation of individual side chains of membrane-binding peptides.

1. Selective incorporation of nitrile-based infrared probes into proteins via cysteine alkylation.

2. Investigation of an unnatural amino acid for use as a resonance Raman probe: Detection limits, solvent and temperature dependence of the νC≡N band of 4-cyanophenylalanine.

3. Site-Specific Spectroscopic Reporters of the Local Electric Field, Hydration, Structure, and Dynamics of Biomolecules.

4. Simulation protocols for coherent femtosecond vibrational spectra of peptides.

5. Computational design and experimental characterization of peptides intended for pH-dependent membrane insertion and pore formation.

6. Computational Modeling of the Nitrile Stretching Vibration of 5-Cyanoindole in Water.

7. Vibrational stark effect probes for nucleic acids.

8. The effects of alpha-helical structure and cyanylated cysteine on each other.

9. Cyanylated Cysteine: A Covalently Attached Vibrational Probe of Protein-Lipid Contacts.

10. Applications of 2D IR spectroscopy to peptides, proteins, and hydrogen-bond dynamics.