Literature DB >> 15089713

Crystallization of a globular protein in lipid cubic phase.

S Tanaka1, S U Egelhaaf, W C K Poon.   

Abstract

We studied the crystallization of a globular protein, lysozyme, in the cubic phase of the lipid mono-olein. The solubility of lysozyme in salt solution decreased by a factor of approximately 4 when confined in cubic phase. Monte Carlo simulations and calculations show that this can be explained by the confinement of lysozyme molecules to the narrow water cells in the cubic phase.

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Year:  2004        PMID： 15089713     DOI： 10.1103/PhysRevLett.92.128102

Source DB:  PubMed          Journal:  Phys Rev Lett        ISSN： 0031-9007            Impact factor:   9.161


  4 in total

1.  The lipid cubic phase or in meso method for crystallizing proteins. Bushings for better manual dispensing.

Authors:  Martin Caffrey; Robert Eifert; Dianfan Li; Nicole Howe
Journal:  J Appl Crystallogr       Date:  2014-08-16       Impact factor: 3.304

2.  A fast, simple and robust protocol for growing crystals in the lipidic cubic phase.

Authors:  Margaret Aherne; Joseph A Lyons; Martin Caffrey
Journal:  J Appl Crystallogr       Date:  2012-10-10       Impact factor: 3.304

Review 3.  A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.

Authors:  Martin Caffrey
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2015-01-01       Impact factor: 1.056

4.  In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells.

Authors:  Arjen J Jakobi; Daniel M Passon; Kèvin Knoops; Francesco Stellato; Mengning Liang; Thomas A White; Thomas Seine; Marc Messerschmidt; Henry N Chapman; Matthias Wilmanns
Journal:  IUCrJ       Date:  2016-01-12       Impact factor: 4.769
  4 in total

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