Literature DB >> 15080672

Tunneling and coupled motion in the Escherichia coli dihydrofolate reductase catalysis.

R Steven Sikorski1, Lin Wang, Kelli A Markham, P T Ravi Rajagopalan, Stephen J Benkovic, Amnon Kohen.   

Abstract

H-transfer was studied in the complex kinetic cascade of dihydrofolate reductase. Intrinsic kinetic isotope effects, their temperature dependence, and other temperature-dependent parameters indicated H-tunneling, but no 1 degrees to 2 degrees coupled motion. The data also suggested environmentally coupled tunneling and commitment to catalysis on pre-steady-state isotope effects.

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Year:  2004        PMID： 15080672     DOI： 10.1021/ja031683w

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  80 in total

1.  Temperature dependence of protein motions in a thermophilic dihydrofolate reductase and its relationship to catalytic efficiency.

Authors:  Olayinka A Oyeyemi; Kevin M Sours; Thomas Lee; Katheryn A Resing; Natalie G Ahn; Judith P Klinman
Journal:  Proc Natl Acad Sci U S A       Date:  2010-05-13       Impact factor: 11.205

2.  Taking Ockham's razor to enzyme dynamics and catalysis.

Authors:  David R Glowacki; Jeremy N Harvey; Adrian J Mulholland
Journal:  Nat Chem       Date:  2012-01-29       Impact factor: 24.427

3.  Evidence that a 'dynamic knockout' in Escherichia coli dihydrofolate reductase does not affect the chemical step of catalysis.

Authors:  E Joel Loveridge; Enas M Behiry; Jiannan Guo; Rudolf K Allemann
Journal:  Nat Chem       Date:  2012-03-11       Impact factor: 24.427

4.  Defining the role of active-site loop fluctuations in dihydrofolate reductase catalysis.

Authors:  Dan McElheny; Jason R Schnell; Jonathan C Lansing; H Jane Dyson; Peter E Wright
Journal:  Proc Natl Acad Sci U S A       Date:  2005-03-28       Impact factor: 11.205

5.  Nonperfect synchronization of reaction center rehybridization in the transition state of the hydride transfer catalyzed by dihydrofolate reductase.

Authors:  Jingzhi Pu; Shuhua Ma; Mireia Garcia-Viloca; Jiali Gao; Donald G Truhlar; Amnon Kohen
Journal:  J Am Chem Soc       Date:  2005-10-26       Impact factor: 15.419

6.  The role of enzyme dynamics and tunnelling in catalysing hydride transfer: studies of distal mutants of dihydrofolate reductase.

Authors:  Lin Wang; Nina M Goodey; Stephen J Benkovic; Amnon Kohen
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2006-08-29       Impact factor: 6.237

7.  Linking protein structure and dynamics to catalysis: the role of hydrogen tunnelling.

Authors:  Judith P Klinman
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2006-08-29       Impact factor: 6.237

8.  Computational approach for ranking mutant enzymes according to catalytic reaction rates.

Authors:  Malika Kumarasiri; Gregory A Baker; Alexander V Soudackov; Sharon Hammes-Schiffer
Journal:  J Phys Chem B       Date:  2009-03-19       Impact factor: 2.991

9.  Biophysical Characterization of a Disabled Double Mutant of Soybean Lipoxygenase: The "Undoing" of Precise Substrate Positioning Relative to Metal Cofactor and an Identified Dynamical Network.

Authors:  Shenshen Hu; Adam R Offenbacher; Erin M Thompson; Christine L Gee; Jarett Wilcoxen; Cody A M Carr; Daniil M Prigozhin; Vanessa Yang; Tom Alber; R David Britt; James S Fraser; Judith P Klinman
Journal:  J Am Chem Soc       Date:  2019-01-15       Impact factor: 15.419

10.  Kinetic isotope effects on aromatic and benzylic hydroxylation by Chromobacterium violaceum phenylalanine hydroxylase as probes of chemical mechanism and reactivity.

Authors:  Aram J Panay; Paul F Fitzpatrick
Journal:  Biochemistry       Date:  2008-09-26       Impact factor: 3.162
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