Literature DB >> 15071047

Nested PCR assay for detection of Leishmania donovani in slit aspirates from post-kala-azar dermal Leishmaniasis Lesions.

Gannavaram Sreenivas1, N A Ansari, Joginder Kataria, Poonam Salotra.   

Abstract

A nested PCR assay to detect parasite DNA in slit aspirates from skin lesions of patients with post-kala-azar dermal lesihmaniasis (PKDL) is described. PCR results were positive in 27 of 29 (93%) samples by nested PCR assay, while only 20 of 29 (69%) were positive in a primary PCR assay. The nested PCR assay allowed reliable diagnosis of PKDL in a noninvasive manner.

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Year:  2004        PMID： 15071047      PMCID： PMC387583          DOI： 10.1128/JCM.42.4.1777-1778.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  Post-kala-azar dermal Leishmaniasis in an HIV-patient.

Authors:  L Rios-Buceta; G F Buezo; P F Peñas; E Dauden-Tello; M Aragües-Montañes; J Fraga-Fernandez; A Garcia-Diez
Journal:  Int J Dermatol       Date:  1996-04       Impact factor: 2.736

2.  Development of a species-specific PCR assay for detection of Leishmania donovani in clinical samples from patients with kala-azar and post-kala-azar dermal leishmaniasis.

Authors:  P Salotra; G Sreenivas; G P Pogue; N Lee; H L Nakhasi; V Ramesh; N S Negi
Journal:  J Clin Microbiol       Date:  2001-03       Impact factor: 5.948

Review 3.  Post-kala-azar dermal leishmaniasis.

Authors:  V Ramesh; A Mukherjee
Journal:  Int J Dermatol       Date:  1995-02       Impact factor: 2.736

4.  Post-kala-azar dermal leishmaniasis associated with AIDS.

Authors:  Achiléa Bittencourt; Nancy Silva; Andréa Straatmann; Victor Luiz Correia Nunes; Ivonise Follador; Roberto Badaró
Journal:  Braz J Infect Dis       Date:  2003-11-03       Impact factor: 1.949

5.  Enzymatic amplification of mini-exon-derived RNA gene spacers of Leishmania donovani: primers and probes for DNA diagnosis.

Authors:  M Q Hassan; A Ghosh; S S Ghosh; M Gupta; D Basu; K K Mallik; S Adhya
Journal:  Parasitology       Date:  1993-12       Impact factor: 3.234

6.  Development and application of the polymerase chain reaction for the detection and identification of Leishmania parasites in clinical material.

Authors:  S E Meredith; E E Zijlstra; G J Schoone; C C Kroon; G J van Eys; K U Schaeffer; A M el-Hassan; P G Lawyer
Journal:  Arch Inst Pasteur Tunis       Date:  1993 Jul-Oct

7.  Use of PCR for diagnosis of post-kala-azar dermal leishmaniasis.

Authors:  O F Osman; L Oskam; N C Kroon; G J Schoone; E T Khalil; A M El-Hassan; E E Zijlstra; P A Kager
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

8.  Post kala-azar dermal leishmaniasis: a neglected aspect of kala-azar control programmes.

Authors:  C P Thakur; K Kumar
Journal:  Ann Trop Med Parasitol       Date:  1992-08

9.  Parasite detection in patients with post kala-azar dermal leishmaniasis in India: a comparison between molecular and immunological methods.

Authors:  P Salotra; G Sreenivas; K R Beena; A Mukherjee; V Ramesh
Journal:  J Clin Pathol       Date:  2003-11       Impact factor: 3.411

Review 10.  Post-kala-azar dermal leishmaniasis.

Authors:  E E Zijlstra; A M Musa; E A G Khalil; I M el-Hassan; A M el-Hassan
Journal:  Lancet Infect Dis       Date:  2003-02       Impact factor: 25.071
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  16 in total

Review 1.  Diagnosis of visceral leishmaniasis: developments over the last decade.

Authors:  Gurumurthy Srividya; Arpita Kulshrestha; Ruchi Singh; Poonam Salotra
Journal:  Parasitol Res       Date:  2011-11-09       Impact factor: 2.289

2.  Serological and Molecular Analysis of Leishmania Infection in Healthy Individuals from Two Districts of West Bengal, India, Endemic for Visceral Leishmaniasis.

Authors:  Himanshu Kaushal; Sujit Kumar Bhattacharya; Sandeep Verma; Poonam Salotra
Journal:  Am J Trop Med Hyg       Date:  2017-06       Impact factor: 2.345

3.  Comparative evaluation of PCR and imprint smear microscopy analyses of skin biopsy specimens in diagnosis of macular, papular, and mixed papulo-nodular lesions of post-kala-azar dermal leishmaniasis.

Authors:  Neena Verma; Dharmendra Singh; Krishna Pandey; Vidya Nand Rabi Das; Chandra Shekhar Lal; Rakesh Bihari Verma; Prabhat Kumar Sinha; Pradeep Das
Journal:  J Clin Microbiol       Date:  2013-09-25       Impact factor: 5.948

4.  PKDL--A Silent Parasite Pool for Transmission of Leishmaniasis in Kala-azar Endemic Areas of Malda District, West Bengal, India.

Authors:  Swagata Ganguly; Pabitra Saha; Moytrey Chatterjee; Surajit Roy; Tamal Kanti Ghosh; Subhasish K Guha; Pratip K Kundu; Dilip K Bera; Nandita Basu; Ardhendu K Maji
Journal:  PLoS Negl Trop Dis       Date:  2015-10-20

5.  Kala-azar and post-kala-azar dermal leishmaniasis, Assam, India.

Authors:  Abdul Mabood Khan; Prafulla Dutta; Siraj Ahmed Khan; Swaroop Kumar Baruah; Dina Raja; Kamal Khound; Jagadish Mahanta
Journal:  Emerg Infect Dis       Date:  2014-03       Impact factor: 6.883

6.  Development of a rapid loop-mediated isothermal amplification assay for diagnosis and assessment of cure of Leishmania infection.

Authors:  Sandeep Verma; Ruchi Singh; Vanila Sharma; Ram Avtar Bumb; Narendra Singh Negi; V Ramesh; Poonam Salotra
Journal:  BMC Infect Dis       Date:  2017-03-23       Impact factor: 3.090

7.  Real-time PCR in detection and quantitation of Leishmania donovani for the diagnosis of Visceral Leishmaniasis patients and the monitoring of their response to treatment.

Authors:  Faria Hossain; Prakash Ghosh; Md Anik Ashfaq Khan; Malcolm S Duthie; Aarthy C Vallur; Alessandro Picone; Randall F Howard; Steven G Reed; Dinesh Mondal
Journal:  PLoS One       Date:  2017-09-28       Impact factor: 3.240

Review 8.  Post kala-azar dermal leishmaniasis: A threat to elimination program.

Authors:  Mallikarjuna Rao Gedda; Bhawana Singh; Dhiraj Kumar; Abhishek Kumar Singh; Prasoon Madhukar; Shreya Upadhyay; Om Prakash Singh; Shyam Sundar
Journal:  PLoS Negl Trop Dis       Date:  2020-07-02

9.  Validation of SYBR green I based closed tube loop mediated isothermal amplification (LAMP) assay and simplified direct-blood-lysis (DBL)-LAMP assay for diagnosis of visceral leishmaniasis (VL).

Authors:  Keerti Kaumudee Dixit; Sandeep Verma; Om Prakash Singh; Dharmendra Singh; Akhil Pratap Singh; Ratan Gupta; Narendra Singh Negi; Pradeep Das; Shyam Sundar; Ruchi Singh; Poonam Salotra
Journal:  PLoS Negl Trop Dis       Date:  2018-11-15

10.  Evaluation of Real-time PCR for Diagnosis of Post-Kala-azar Dermal Leishmaniasis in Endemic Foci of Bangladesh.

Authors:  Prakash Ghosh; Md Golam Hasnain; Faria Hossain; Md Anik Ashfaq Khan; Rajashree Chowdhury; Khaledul Faisal; Moshtaq Ahmed Mural; James Baker; Rupen Nath; Debashis Ghosh; Shomik Maruf; Mohammad Sohel Shomik; Rashidul Haque; Greg Matlashewski; Shinjiro Hamano; Malcolm S Duthie; Dinesh Mondal
Journal:  Open Forum Infect Dis       Date:  2018-09-15       Impact factor: 3.835
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