Literature DB >> 15071040

First international quality assurance study on the rapid detection of viral agents of bioterrorism.

Matthias Niedrig1, Herbert Schmitz, Stephan Becker, Stephan Günther, Jan ter Meulen, Herman Meyer, Heinz Ellerbrok, Andreas Nitsche, Hans R Gelderblom, Christian Drosten.   

Abstract

We have conducted an international quality assurance study of filovirus, Lassa virus, and orthopox virus PCR with 24 participants. Of the participating laboratories, 45.8 and 66.7% detected virus in all plasma samples, which contained > or = 5,000 and > or = 100,000 copies per ml, respectively. Sensitivity levels were not significantly different between viruses. False-negative results were attributable to a lack of sensitivity.

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Year:  2004        PMID: 15071040      PMCID: PMC387573          DOI: 10.1128/JCM.42.4.1753-1755.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  8 in total

1.  Multicenter proficiency testing of nucleic acid amplification methods for the detection of enteroviruses.

Authors:  K E Van Vliet; P Muir; J M Echevarria; P E Klapper; G M Cleator; A M Van Loon
Journal:  J Clin Microbiol       Date:  2001-09       Impact factor: 5.948

Review 2.  Biological warfare and bioterrorism.

Authors:  Nicholas J Beeching; David A B Dance; Alastair R O Miller; Robert C Spencer
Journal:  BMJ       Date:  2002-02-09

3.  Detection of smallpox virus DNA by LightCycler PCR.

Authors:  Mark J Espy; Franklin R Cockerill III; Richard F Meyer; Michael D Bowen; Gregory A Poland; Ted L Hadfield; Thomas F Smith
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

Review 4.  Molecular diagnostics of viral hemorrhagic fevers.

Authors:  Christian Drosten; Beate M Kümmerer; Herbert Schmitz; Stephan Günther
Journal:  Antiviral Res       Date:  2003-01       Impact factor: 5.970

5.  False-negative results of PCR assay with plasma of patients with severe viral hemorrhagic fever.

Authors:  Christian Drosten; Marcus Panning; Stephan Guenther; Herbert Schmitz
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

6.  Rapid and simple method for purification of nucleic acids.

Authors:  R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

7.  Public health assessment of potential biological terrorism agents.

Authors:  Lisa D Rotz; Ali S Khan; Scott R Lillibridge; Stephen M Ostroff; James M Hughes
Journal:  Emerg Infect Dis       Date:  2002-02       Impact factor: 6.883

8.  Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR.

Authors:  Christian Drosten; Stephan Göttig; Stefan Schilling; Marcel Asper; Marcus Panning; Herbert Schmitz; Stephan Günther
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

  8 in total
  18 in total

Review 1.  Laboratory diagnostic systems for Ebola and Marburg hemorrhagic fevers developed with recombinant proteins.

Authors:  Masayuki Saijo; Masahiro Niikura; Tetsuro Ikegami; Ichiro Kurane; Takeshi Kurata; Shigeru Morikawa
Journal:  Clin Vaccine Immunol       Date:  2006-04

2.  Follow-up on diagnostic proficiency of laboratories equipped to perform orthopoxvirus detection and quantification by PCR: the second international external quality assurance study.

Authors:  Matthias Niedrig; Hermann Meyer; Marcus Panning; Christian Drosten
Journal:  J Clin Microbiol       Date:  2006-04       Impact factor: 5.948

Review 3.  Diagnosis of Ebola Virus Disease: Past, Present, and Future.

Authors:  M Jana Broadhurst; Tim J G Brooks; Nira R Pollock
Journal:  Clin Microbiol Rev       Date:  2016-10       Impact factor: 26.132

4.  Use of internally controlled real-time genome amplification for detection of variola virus and other orthopoxviruses infecting humans.

Authors:  C G Fedele; A Negredo; F Molero; M P Sánchez-Seco; A Tenorio
Journal:  J Clin Microbiol       Date:  2006-10-25       Impact factor: 5.948

5.  Real-time PCR system for detection of orthopoxviruses and simultaneous identification of smallpox virus.

Authors:  Victoria A Olson; Thomas Laue; Miriam T Laker; Igor V Babkin; Christian Drosten; Sergei N Shchelkunov; Matthias Niedrig; Inger K Damon; Hermann Meyer
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

6.  The use of a mobile laboratory unit in support of patient management and epidemiological surveillance during the 2005 Marburg Outbreak in Angola.

Authors:  Allen Grolla; Steven M Jones; Lisa Fernando; James E Strong; Ute Ströher; Peggy Möller; Janusz T Paweska; Felicity Burt; Pedro Pablo Palma; Armand Sprecher; Pierre Formenty; Cathy Roth; Heinz Feldmann
Journal:  PLoS Negl Trop Dis       Date:  2011-05-24

7.  Detection of infectious poxvirus particles.

Authors:  Andreas Nitsche; Daniel Stern; Heinz Ellerbrok; Georg Pauli
Journal:  Emerg Infect Dis       Date:  2006-07       Impact factor: 6.883

8.  SARS molecular detection external quality assurance.

Authors:  Christian Drosten; Hans Wilhelm Doerr; Wilina Lim; Klaus Stöhr; Matthias Niedrig
Journal:  Emerg Infect Dis       Date:  2004-12       Impact factor: 6.883

9.  International external quality assessment study for molecular detection of Lassa virus.

Authors:  Sergejs Nikisins; Toni Rieger; Pranav Patel; Rolf Müller; Stephan Günther; Matthias Niedrig
Journal:  PLoS Negl Trop Dis       Date:  2015-05-21

10.  Coordinated implementation of chikungunya virus reverse transcription-PCR.

Authors:  Marcus Panning; Remi N Charrel; Oliver Donoso Mantke; Oliver D Mantke; Olfert Landt; Matthias Niedrig; Christian Drosten
Journal:  Emerg Infect Dis       Date:  2009-03       Impact factor: 6.883

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