Literature DB >> 15066851

Targeted isolation of a designated region of the Bacillus subtilis genome by recombinational transfer.

Satoshi Tomita1, Kenji Tsuge, Yo Kikuchi, Mitsuhiro Itaya.   

Abstract

A method for positional cloning of the Bacillus subtilis genome was developed. The method requires a set of two small DNA fragments that flank the region to be copied. A 38-kb segment that carries genes ppsABCDE encoding five enzymes for antibiotic plipastatin synthesis and another genome locus as large as 100 kb including one essential gene were examined for positional cloning. The positional cloning vector for ppsABCDE was constructed using a B. subtilis low-copy-number plasmid that faithfully copied the precise length of the 38-kb DNA in vivo via the recombinational transfer system of this bacterium. Structure of the copied DNA was confirmed by restriction enzyme analyses. Furthermore, the unaltered structure of the 38-kb DNA was demonstrated by complementation of a ppsABCDE deletion mutant.

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Year:  2004        PMID： 15066851      PMCID： PMC383163          DOI： 10.1128/AEM.70.4.2508-2513.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  18 in total

1.  Essential Bacillus subtilis genes.

Authors:  K Kobayashi; S D Ehrlich; A Albertini; G Amati; K K Andersen; M Arnaud; K Asai; S Ashikaga; S Aymerich; P Bessieres; F Boland; S C Brignell; S Bron; K Bunai; J Chapuis; L C Christiansen; A Danchin; M Débarbouille; E Dervyn; E Deuerling; K Devine; S K Devine; O Dreesen; J Errington; S Fillinger; S J Foster; Y Fujita; A Galizzi; R Gardan; C Eschevins; T Fukushima; K Haga; C R Harwood; M Hecker; D Hosoya; M F Hullo; H Kakeshita; D Karamata; Y Kasahara; F Kawamura; K Koga; P Koski; R Kuwana; D Imamura; M Ishimaru; S Ishikawa; I Ishio; D Le Coq; A Masson; C Mauël; R Meima; R P Mellado; A Moir; S Moriya; E Nagakawa; H Nanamiya; S Nakai; P Nygaard; M Ogura; T Ohanan; M O'Reilly; M O'Rourke; Z Pragai; H M Pooley; G Rapoport; J P Rawlins; L A Rivas; C Rivolta; A Sadaie; Y Sadaie; M Sarvas; T Sato; H H Saxild; E Scanlan; W Schumann; J F M L Seegers; J Sekiguchi; A Sekowska; S J Séror; M Simon; P Stragier; R Studer; H Takamatsu; T Tanaka; M Takeuchi; H B Thomaides; V Vagner; J M van Dijl; K Watabe; A Wipat; H Yamamoto; M Yamamoto; Y Yamamoto; K Yamane; K Yata; K Yoshida; H Yoshikawa; U Zuber; N Ogasawara
Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-07       Impact factor: 11.205

2.  REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

Authors:  C Anagnostopoulos; J Spizizen
Journal:  J Bacteriol       Date:  1961-05       Impact factor: 3.490

3.  TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.

Authors:  J Spizizen
Journal:  Proc Natl Acad Sci U S A       Date:  1958-10-15       Impact factor: 11.205

4.  Genetic transfer of large DNA inserts to designated loci of the Bacillus subtilis 168 genome.

Authors:  M Itaya
Journal:  J Bacteriol       Date:  1999-02       Impact factor: 3.490

5.  Experimental surgery to create subgenomes of Bacillus subtilis 168.

Authors:  M Itaya; T Tanaka
Journal:  Proc Natl Acad Sci U S A       Date:  1997-05-13       Impact factor: 11.205

6.  Fate of unstable Bacillus subtilis subgenome: re-integration and amplification in the main genome.

Authors:  M Itaya; T Tanaka
Journal:  FEBS Lett       Date:  1999-04-09       Impact factor: 4.124

7.  The complete genome sequence of the gram-positive bacterium Bacillus subtilis.

Authors:  F Kunst; N Ogasawara; I Moszer; A M Albertini; G Alloni; V Azevedo; M G Bertero; P Bessières; A Bolotin; S Borchert; R Borriss; L Boursier; A Brans; M Braun; S C Brignell; S Bron; S Brouillet; C V Bruschi; B Caldwell; V Capuano; N M Carter; S K Choi; J J Cordani; I F Connerton; N J Cummings; R A Daniel; F Denziot; K M Devine; A Düsterhöft; S D Ehrlich; P T Emmerson; K D Entian; J Errington; C Fabret; E Ferrari; D Foulger; C Fritz; M Fujita; Y Fujita; S Fuma; A Galizzi; N Galleron; S Y Ghim; P Glaser; A Goffeau; E J Golightly; G Grandi; G Guiseppi; B J Guy; K Haga; J Haiech; C R Harwood; A Hènaut; H Hilbert; S Holsappel; S Hosono; M F Hullo; M Itaya; L Jones; B Joris; D Karamata; Y Kasahara; M Klaerr-Blanchard; C Klein; Y Kobayashi; P Koetter; G Koningstein; S Krogh; M Kumano; K Kurita; A Lapidus; S Lardinois; J Lauber; V Lazarevic; S M Lee; A Levine; H Liu; S Masuda; C Mauël; C Médigue; N Medina; R P Mellado; M Mizuno; D Moestl; S Nakai; M Noback; D Noone; M O'Reilly; K Ogawa; A Ogiwara; B Oudega; S H Park; V Parro; T M Pohl; D Portelle; S Porwollik; A M Prescott; E Presecan; P Pujic; B Purnelle; G Rapoport; M Rey; S Reynolds; M Rieger; C Rivolta; E Rocha; B Roche; M Rose; Y Sadaie; T Sato; E Scanlan; S Schleich; R Schroeter; F Scoffone; J Sekiguchi; A Sekowska; S J Seror; P Serror; B S Shin; B Soldo; A Sorokin; E Tacconi; T Takagi; H Takahashi; K Takemaru; M Takeuchi; A Tamakoshi; T Tanaka; P Terpstra; A Togoni; V Tosato; S Uchiyama; M Vandebol; F Vannier; A Vassarotti; A Viari; R Wambutt; H Wedler; T Weitzenegger; P Winters; A Wipat; H Yamamoto; K Yamane; K Yasumoto; K Yata; K Yoshida; H F Yoshikawa; E Zumstein; H Yoshikawa; A Danchin
Journal:  Nature       Date:  1997-11-20       Impact factor: 49.962

8.  Molecular archaeology of the Escherichia coli genome.

Authors:  J G Lawrence; H Ochman
Journal:  Proc Natl Acad Sci U S A       Date:  1998-08-04       Impact factor: 11.205

9.  One step assembly of multiple DNA fragments with a designed order and orientation in Bacillus subtilis plasmid.

Authors:  Kenji Tsuge; Kuniko Matsui; Mitsuhiro Itaya
Journal:  Nucleic Acids Res       Date:  2003-11-01       Impact factor: 16.971

10.  Toward a bacterial genome technology: integration of the Escherichia coli prophage lambda genome into the Bacillus subtilis 168 chromosome.

Authors:  M Itaya
Journal:  Mol Gen Genet       Date:  1995-07-22
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