Literature DB >> 1506678

A simple enhancement method for the silver-gold-intensified diaminobenzidine reaction in the light microscopic immunoperoxidase technique.

S Goto1, S Nagahiro, Y Ushio, W Hofer.   

Abstract

We describe a simple and sensitive method for enhancement of the silver-gold-intensified 3,3'-diaminobenzidine (DAB) reaction demonstrating peroxidase activity. After completing silver-gold intensification of the preparations immunostained by the avidin-biotin-peroxidase method with DAB as the chromogen, the preparations were immersed in a solution containing uranyl nitrate. This new method appeared to increase the sensitivity by at least one order of magnitude as compared with silver-gold intensification alone.

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Year:  1992        PMID： 1506678     DOI： 10.1177/40.9.1506678

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  4 in total

Review 1.  Antigen retrieval, signal amplification and intensification in immunohistochemistry.

Authors:  M Werner; R Von Wasielewski; P Komminoth
Journal:  Histochem Cell Biol       Date:  1996-04       Impact factor: 4.304

2.  A sensitive post-DAB enhancement technique for demonstration of iron in the central nervous system.

Authors:  T Moos; K Møllgård
Journal:  Histochemistry       Date:  1993-06

3.  Striatonigral involvement following transient focal cerebral ischemia in the rats: an immunohistochemical study on a reversible ischemia model.

Authors:  S Goto; S Nagahiro; K Korematsu; Y Ushio
Journal:  Acta Neuropathol       Date:  1993       Impact factor: 17.088

4.  Development of a highly sensitive immunohistochemical method to detect neurochemical molecules in formalin-fixed and paraffin-embedded tissues from autopsied human brains.

Authors:  Satoshi Goto; Ryoma Morigaki; Shinya Okita; Shinji Nagahiro; Ryuji Kaji
Journal:  Front Neuroanat       Date:  2015-03-03       Impact factor: 3.856
  4 in total

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