| Literature DB >> 15051398 |
Meng Fu1, Albert Koulman, Marion van Rijssel, Arne Lützen, M Karin de Boer, Monika R Tyl, Gerd Liebezeit.
Abstract
The molecular structures of the three main haemolytic compounds (Fj1, Fj2 and Fj3) isolated from the ichthyotoxic microalgal species Fibrocapsa japonica have been investigated by NMR, LC-ESI-MS, ESI-MS-MS, IR, GC-MS and GC-HRMS methods. They are polyunsaturated fatty acids which we identified as: 6,9,12,15-octadecatetraenoic acid (OTA, C18:4omega3), 5,8,11,14,17-eicosapentaenoic acid (EPA, C20:5omega3) and 5,8,11,14-eicosatetraenoic acid (arachidonic acid AA, C20:4omega6). The identity of the latter two was confirmed on the basis of commercial standards (C20:5omega3 and C20:4omega6). Both displayed the same spectroscopic and chromatographic characteristics as Fj2 and Fj3 and had a similar strong haemolytic effect. We propose that when F. japonica cells accumulate in fish gills during blooms these compounds could be the cause of icthyotoxicity.Entities:
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Year: 2004 PMID： 15051398 DOI： 10.1016/j.toxicon.2003.09.012
Source DB: PubMed Journal: Toxicon ISSN： 0041-0101 Impact factor: 3.033