J Laitakari1, V Näyhä, F Stenbäck. 1. Department of Pathology, University of Oulu, PO Box 5000, Oulu 90014, Finland.
Abstract
AIMS: Angiogenesis and vessel organisation in laryngeal tumour development and progression were examined to determine characteristics of biological and clinical relevance. METHODS: Automated quantitative image analysis was performed on 1451 factor VIII (FVIII) associated blood vessels with regard to occurrence, structure, size, shape, and staining intensity, in addition to vessel direction. RESULTS: Vessel numbers were increased in preneoplastic states and severe dysplasia, in addition to squamous cell carcinomas, being greater in poorly differentiated carcinomas. Small regular vessels predominated in benign conditions and large, irregular vessels in malignant neoplasms. Vessel distribution was related to degree of differentiation in squamous cell carcinomas, with circumferential angiogenesis occurring in well differentiated neoplasms, directional angiogenesis in moderately differentiated tumours, and aberrant angiogenesis in less well differentiated neoplasms. Alterations in vessel shape increased significantly with increasing degree of malignancy. Comparing the characteristics of individual vessels showed vessel shape abnormalities and the intensity of FVIII staining to increase with vessel size. CONCLUSIONS: Increased angiogenesis was an early event in laryngeal tumour development, with vessel structure, size, and shape related to the tumour growth pattern and behaviour.
AIMS: Angiogenesis and vessel organisation in laryngeal tumour development and progression were examined to determine characteristics of biological and clinical relevance. METHODS: Automated quantitative image analysis was performed on 1451 factor VIII (FVIII) associated blood vessels with regard to occurrence, structure, size, shape, and staining intensity, in addition to vessel direction. RESULTS: Vessel numbers were increased in preneoplastic states and severe dysplasia, in addition to squamous cell carcinomas, being greater in poorly differentiated carcinomas. Small regular vessels predominated in benign conditions and large, irregular vessels in malignant neoplasms. Vessel distribution was related to degree of differentiation in squamous cell carcinomas, with circumferential angiogenesis occurring in well differentiated neoplasms, directional angiogenesis in moderately differentiated tumours, and aberrant angiogenesis in less well differentiated neoplasms. Alterations in vessel shape increased significantly with increasing degree of malignancy. Comparing the characteristics of individual vessels showed vessel shape abnormalities and the intensity of FVIII staining to increase with vessel size. CONCLUSIONS: Increased angiogenesis was an early event in laryngeal tumour development, with vessel structure, size, and shape related to the tumour growth pattern and behaviour.
Authors: K M McLaren; R A Burnett; J R Goodlad; S R Howatson; S Lang; F D Lee; A M Lessells; S Ogston; A J Robertson; J G Simpson; G D Smith; H B Tavadia; F Walker Journal: Histopathology Date: 2000-11 Impact factor: 5.087
Authors: Lorelei A Mucci; Anna Powolny; Edward Giovannucci; Zhiming Liao; Stacey A Kenfield; Rulong Shen; Meir J Stampfer; Steven K Clinton Journal: J Clin Oncol Date: 2009-10-26 Impact factor: 44.544
Authors: Mukund Seshadri; Mihai Merzianu; Haikuo Tang; Nestor R Rigual; Maureen Sullivan; Thom R Loree; Saurin R Popat; Elizabeth A Repasky; Bonnie L Hylander Journal: Cancer Biol Ther Date: 2009-12-19 Impact factor: 4.742
Authors: Lars Tore Gyland Mikalsen; Hari Prasad Dhakal; Øyvind S Bruland; Bjørn Naume; Elin Borgen; Jahn M Nesland; Dag Rune Olsen Journal: PLoS One Date: 2013-10-11 Impact factor: 3.240