Literature DB >> 15025444

Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins.

Chenjie Xu1, Keming Xu, Hongwei Gu, Xiaofen Zhong, Zhihong Guo, Rongkun Zheng, Xixiang Zhang, Bing Xu.   

Abstract

Using Nalpha,Nalpha-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt-NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.

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Year:  2004        PMID： 15025444     DOI： 10.1021/ja031776d

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  32 in total

Review 1.  Recent Developments in Magnetic Diagnostic Systems.

Authors:  Hakho Lee; Tae-Hyun Shin; Jinwoo Cheon; Ralph Weissleder
Journal:  Chem Rev       Date:  2015-08-10       Impact factor: 60.622

2.  Synthesis and characterization of multifunctional silica core-shell nanocomposites with magnetic and fluorescent functionalities.

Authors:  Zhiya Ma; Dosi Dosev; Mikaela Nichkova; Randy K Dumas; Shirley J Gee; Bruce D Hammock; Kai Liu; Ian M Kennedy
Journal:  J Magn Magn Mater       Date:  2009-05-01       Impact factor: 2.993

3.  Efficient and stable display of functional proteins on bacterial magnetic particles using mms13 as a novel anchor molecule.

Authors:  Tomoko Yoshino; Tadashi Matsunaga
Journal:  Appl Environ Microbiol       Date:  2006-01       Impact factor: 4.792

4.  Au-Fe3O4 dumbbell nanoparticles as dual-functional probes.

Authors:  Chenjie Xu; Jin Xie; Don Ho; Chao Wang; Nathan Kohler; Edward G Walsh; Jeffrey R Morgan; Y Eugene Chin; Shouheng Sun
Journal:  Angew Chem Int Ed Engl       Date:  2008       Impact factor: 15.336

5.  Microgels for efficient protein purification.

Authors:  Boaz Mizrahi; Silvia Irusta; Marshall McKenna; Cristina Stefanescu; Liron Yedidsion; Myatnoezin Myint; Robert Langer; Daniel S Kohane
Journal:  Adv Mater       Date:  2011-08-01       Impact factor: 30.849

Review 6.  Beauty is skin deep: a surface monolayer perspective on nanoparticle interactions with cells and bio-macromolecules.

Authors:  Krishnendu Saha; Avinash Bajaj; Bradley Duncan; Vincent M Rotello
Journal:  Small       Date:  2011-06-14       Impact factor: 13.281

Review 7.  Post-genomics nanotechnology is gaining momentum: nanoproteomics and applications in life sciences.

Authors:  Firas H Kobeissy; Basri Gulbakan; Ali Alawieh; Pierre Karam; Zhiqun Zhang; Joy D Guingab-Cagmat; Stefania Mondello; Weihong Tan; John Anagli; Kevin Wang
Journal:  OMICS       Date:  2014-01-10

8.  One-Pot Green Synthesis and Bioapplication ofl-Arginine-Capped Superparamagnetic Fe3O4 Nanoparticles.

Authors:  Yongchao Lai; Weiwei Yin; Jinting Liu; Rimo Xi; Jinhua Zhan
Journal:  Nanoscale Res Lett       Date:  2009-11-13       Impact factor: 4.703

9.  Effects of proteins from culture medium on surface property of silanes- functionalized magnetic nanoparticles.

Authors:  Zp Chen; Rz Xu; Y Zhang; N Gu
Journal:  Nanoscale Res Lett       Date:  2008-12-09       Impact factor: 4.703

10.  Study on Composition Distribution and Ferromagnetism of Monodisperse FePt Nanoparticles.

Authors:  Hb Wang; H Wang; J Zhang; Fj Yang; Ym Xu; Q Li
Journal:  Nanoscale Res Lett       Date:  2010-02-04       Impact factor: 4.703
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