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Literature DB >> 15024496

Intraoperative dissemination of tumour cells in patients with Ewing tumours detected by RT-PCR.

J G Grohs¹, A Zoubek, D Jugovic, H Kovar, R Windhager.

Abstract

Using reverse transcriptase polymerase chain reaction (RT-PCR) we evaluated the occurrence of tumour-cell ribonucleic acid (RNA) in the blood during surgery in patients with Ewing tumours. The patients received irradiation and chemotherapy according to the protocol of the European Intergroup Cooperative Ewing Sarcoma Study (EICESS) 92. Blood samples were taken from 15 patients. Intra-operative dissemination was found during 2/8 resections but showed no relation to patient survival. At second-look biopsy, detection of tumour-cell RNA was associated with relapse and metastases in 3/4 patients. The results suggest that pre-operative treatment did not completely prevent dissemination of tumour cells during surgery of Ewing tumours.

Entities: Disease Species

Mesh：

Substances：
RNA, Neoplasm

Year: 2004 PMID： 15024496 PMCID： PMC3456931 DOI： 10.1007/s00264-004-0551-4

Source DB: PubMed Journal: Int Orthop ISSN： 0341-2695 Impact factor: 3.075

14 in total

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8. Increased risk of systemic relapses associated with bone marrow micrometastasis and circulating tumor cells in localized ewing tumor.

Authors: Gudrun Schleiermacher; Martine Peter; Odile Oberlin; Thierry Philip; Hervé Rubie; Françoise Mechinaud; Danièle Sommelet-Olive; Judith Landman-Parker; Danièle Bours; Jean Michon; Olivier Delattre
Journal: J Clin Oncol Date: 2003-01-01 Impact factor: 44.544

Intraoperative dissemination of tumour cells in patients with Ewing tumours detected by RT-PCR.

1. Presence of tumor cells in bone marrow but not in blood is associated with adverse prognosis in patients with Ewing's tumor. Société Française d'Oncologie Pédiatrique.

2. Multidisciplinary treatment of primary Ewing's sarcoma of bone. A 6-year experience of a European Cooperative Trial.

3. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

4. Detection and viability of tumor cells in peripheral blood stem cell collections from breast cancer patients using immunocytochemical and clonogenic assay techniques.

5. Detection of tumour cells in peripheral blood and bone marrow from Ewing tumour patients by RT-PCR.

6. Morphological grades of regression in osteosarcoma after polychemotherapy - study COSS 80.

7. Molecular staging of prostate cancer with the use of an enhanced reverse transcriptase-PCR assay.

8. Increased risk of systemic relapses associated with bone marrow micrometastasis and circulating tumor cells in localized ewing tumor.

9. Sensitive detection of occult Ewing's cells by the reverse transcriptase-polymerase chain reaction.

10. Variability of EWS chimaeric transcripts in Ewing tumours: a comparison of clinical and molecular data.

Review 1. The tactics and technique of musculoskeletal biopsy.

Review 2. Comparative characteristics of small cell lung cancer and Ewing's sarcoma: a narrative review.