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Literature DB >> 14990785

A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system.

Abstract

Emulsion formulations used for in vitro compartmentalization (IVC) methods were found to be incompatible with protein expression in the rabbit reticulocyte (RRL) system, causing rapid discoloration and translation shutdown. Here we identify possible causes and describe a novel water-in-oil emulsion which abolished discoloration and allowed high-level in-emulsion expression of active luciferase and human telomerase using the RRL. This novel emulsion greatly expands the range of potential protein targets for IVC.

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Year: 2004 PMID： 14990785 DOI： 10.1093/protein/gzh025

Source DB: PubMed Journal: Protein Eng Des Sel ISSN： 1741-0126 Impact factor: 1.650

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Cited

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Journal: Proc Natl Acad Sci U S A Date: 2005-10-28 Impact factor: 11.205

A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system.

1. Molecular evolution picks up the PACE.

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