BACKGROUND: To investigate the differential expression levels of thymosin beta 10 (T beta 10) and the corresponding changes of actin filament organization in human tumor cell lines with different metastatic potential. METHODS: Four groups of nine human tumor cell lines with different metastatic potential were analyzed for the amount of T beta 10 mRNAs by Northern blot and for their peptide expression levels by immunohistochemistry. The filamentous actin (F-actin) was observed by staining of TRITC-phalloidin to detect changes in actin organization. RESULTS: In comparison with non-/weakly metastatic counterparts, T beta 10 was upregulated in highly metastatic human lung cancer, malignant melanoma and breast cancer cell lines. Staining of TRITC-phalloidin revealed less actin bundles, a fuzzy network of shorter filaments and some F-actin aggregates in the highly metastatic tumor cells. Meanwhile, the actin filaments were robust and orderly arranged in the non-/weakly metastatic cancer cell lines. CONCLUSION: T beta 10 levels correlate positively with the metastatic capacity in human tumors currently examined. The increasing metastatic potential of tumor cells is accompanied by a loss of F-actin, poorly arranged actin skeleton organizations and presence of F-actin aggregates. There is a consistent correlation between the elevated T beta 10 expression and the disrupted actin skeleton.
BACKGROUND: To investigate the differential expression levels of thymosin beta 10 (T beta 10) and the corresponding changes of actin filament organization in humantumor cell lines with different metastatic potential. METHODS: Four groups of nine humantumor cell lines with different metastatic potential were analyzed for the amount of T beta 10 mRNAs by Northern blot and for their peptide expression levels by immunohistochemistry. The filamentous actin (F-actin) was observed by staining of TRITC-phalloidin to detect changes in actin organization. RESULTS: In comparison with non-/weakly metastatic counterparts, T beta 10 was upregulated in highly metastatic humanlung cancer, malignant melanoma and breast cancer cell lines. Staining of TRITC-phalloidin revealed less actin bundles, a fuzzy network of shorter filaments and some F-actin aggregates in the highly metastatic tumor cells. Meanwhile, the actin filaments were robust and orderly arranged in the non-/weakly metastatic cancer cell lines. CONCLUSION: T beta 10 levels correlate positively with the metastatic capacity in humantumors currently examined. The increasing metastatic potential of tumor cells is accompanied by a loss of F-actin, poorly arranged actin skeleton organizations and presence of F-actin aggregates. There is a consistent correlation between the elevated T beta 10 expression and the disrupted actin skeleton.
Authors: M I Vitolo; A E Boggs; R A Whipple; J R Yoon; K Thompson; M A Matrone; E H Cho; E M Balzer; S S Martin Journal: Oncogene Date: 2012-06-11 Impact factor: 9.867
Authors: Min Li; Yuqing Zhang; Qihui Zhai; Louis W Feurino; William E Fisher; Changyi Chen; Qizhi Yao Journal: Cancer Invest Date: 2009-03 Impact factor: 2.176
Authors: Stephanie D Byrum; Signe K Larson; Nathan L Avaritt; Linley E Moreland; Samuel G Mackintosh; Wang L Cheung; Alan J Tackett Journal: J Proteomics Bioinform Date: 2013-03-01
Authors: Anna A Mourskaia; Eitan Amir; Zhifeng Dong; Kerstin Tiedemann; Sean Cory; Atilla Omeroglu; Nicholas Bertos; Véronique Ouellet; Mark Clemons; George L Scheffer; Morag Park; Michael Hallett; Svetlana V Komarova; Peter M Siegel Journal: Breast Cancer Res Date: 2012-11-22 Impact factor: 6.466