BACKGROUND: Prostate-specific antigen (PSA) is a serine protease with highly prostate-specific expression and an important marker for prostate cancer. We have previously identified novel PSA-binding peptides that enhance the enzymatic activity of PSA when produced as fusion proteins. METHOD: PSA-binding peptides and derivatives with a spacer were chemically synthesized and used to prepare an affinity column, which was used to fractionate PSA in seminal plasma, serum, and LNCap cell culture medium. RESULTS: Approximately 67% of seminal plasma PSA bound to the peptide affinity column and was eluted under mild conditions. Eluted PSA was intact and enzymatically active while the unbound fraction mainly contained various nicked forms. ProPSA from LNCap cells bound to the peptide column only after activation by trypsin. CONCLUSIONS: PSA-binding peptides can be used to separate enzymatically active and inactive forms of PSA. Thus the peptides are potentially useful as ligands for development of methods for specific detection of active free PSA. Copyright 2003 Wiley-Liss, Inc.
BACKGROUND:Prostate-specific antigen (PSA) is a serine protease with highly prostate-specific expression and an important marker for prostate cancer. We have previously identified novel PSA-binding peptides that enhance the enzymatic activity of PSA when produced as fusion proteins. METHOD:PSA-binding peptides and derivatives with a spacer were chemically synthesized and used to prepare an affinity column, which was used to fractionate PSA in seminal plasma, serum, and LNCap cell culture medium. RESULTS: Approximately 67% of seminal plasma PSA bound to the peptide affinity column and was eluted under mild conditions. Eluted PSA was intact and enzymatically active while the unbound fraction mainly contained various nicked forms. ProPSA from LNCap cells bound to the peptide column only after activation by trypsin. CONCLUSIONS:PSA-binding peptides can be used to separate enzymatically active and inactive forms of PSA. Thus the peptides are potentially useful as ligands for development of methods for specific detection of active free PSA. Copyright 2003 Wiley-Liss, Inc.
Authors: Kristian Meinander; Miikka Pakkala; Janne Weisell; Ulf-Håkan Stenman; Hannu Koistinen; Ale Närvänen; Erik A A Wallén Journal: ACS Med Chem Lett Date: 2013-12-16 Impact factor: 4.345
Authors: Cheng Sun; Kai-Hung Su; Jason Valentine; Yazmin T Rosa-Bauza; Jonathan A Ellman; Omeed Elboudwarej; Bipasha Mukherjee; Charles S Craik; Marc A Shuman; Fanqing Frank Chen; Xiang Zhang Journal: ACS Nano Date: 2010-02-23 Impact factor: 15.881
Authors: Johanna M Mattsson; Suvi Ravela; Can Hekim; Magnus Jonsson; Johan Malm; Ale Närvänen; Ulf-Håkan Stenman; Hannu Koistinen Journal: PLoS One Date: 2014-09-19 Impact factor: 3.240