OBJECTIVE: To identify stem cells in uteri from the fetal to the postmenopausal period. DESIGN: Descriptive, controlled study. SETTING: Tertiary academic medical center. PATIENT(S): Forty archived uterine endometrial samples. INTERVENTION(S): Immunohistochemistry with c-kit/CD117, CD34, bcl-2, and Ki67. MAIN OUTCOME MEASURE(S): Immunostaining. RESULT(S): C-kit/CD117, CD34, bcl-2, and Ki67 were consistently expressed in the stroma of the basalis layer. Bcl-2 was restricted to the fetal uterine endometrium. CONCLUSION(S): By studying the immunolocalization of stem cell markers (i.e., c-kit, CD34, and bcl-2), we found that stem cells are present continuously in the uterine endometrium from the fetal period. The cells seem to be located mainly in the stroma of the basalis and to be independent of hormonal regulation to avoid cyclic shedding. A small number of latent stem cells dormant in the stroma of the basalis were found to have the potential for mesenchymal differentiation.
OBJECTIVE: To identify stem cells in uteri from the fetal to the postmenopausal period. DESIGN: Descriptive, controlled study. SETTING: Tertiary academic medical center. PATIENT(S): Forty archived uterine endometrial samples. INTERVENTION(S): Immunohistochemistry with c-kit/CD117, CD34, bcl-2, and Ki67. MAIN OUTCOME MEASURE(S): Immunostaining. RESULT(S): C-kit/CD117, CD34, bcl-2, and Ki67 were consistently expressed in the stroma of the basalis layer. Bcl-2 was restricted to the fetal uterine endometrium. CONCLUSION(S): By studying the immunolocalization of stem cell markers (i.e., c-kit, CD34, and bcl-2), we found that stem cells are present continuously in the uterine endometrium from the fetal period. The cells seem to be located mainly in the stroma of the basalis and to be independent of hormonal regulation to avoid cyclic shedding. A small number of latent stem cells dormant in the stroma of the basalis were found to have the potential for mesenchymal differentiation.
Authors: Maria Carolina O Rodrigues; Dmitriy Dmitriev; Antonio Rodrigues; Loren E Glover; Paul R Sanberg; Julie G Allickson; Nicole Kuzmin-Nichols; Naoki Tajiri; Kazutaka Shinozuka; Svitlana Garbuzova-Davis; Yuji Kaneko; Cesar V Borlongan Journal: Interv Med Appl Sci Date: 2012-06
Authors: Paula Gabriela Marin Figueira; Mauricio Simões Abrão; Graciela Krikun; Hugh S Taylor; Hugh Taylor Journal: Ann N Y Acad Sci Date: 2011-03 Impact factor: 5.691
Authors: Irene Cervelló; Claudia Gil-Sanchis; Aymara Mas; Francisco Delgado-Rosas; José Antonio Martínez-Conejero; Amparo Galán; Alicia Martínez-Romero; Sebastian Martínez; Ismael Navarro; Jaime Ferro; José Antonio Horcajadas; Francisco José Esteban; José Enrique O'Connor; Antonio Pellicer; Carlos Simón Journal: PLoS One Date: 2010-06-24 Impact factor: 3.240
Authors: Eva-Katrin Bentz; Marina Kenning; Christian Schneeberger; Andrea Kolbus; Johannes C Huber; Lukas A Hefler; Clemens B Tempfer Journal: Reprod Biol Endocrinol Date: 2010-04-22 Impact factor: 5.211
Authors: Cesar V Borlongan; Yuji Kaneko; Mina Maki; Seong-Jin Yu; Mohammed Ali; Julie G Allickson; Cyndy D Sanberg; Nicole Kuzmin-Nichols; Paul R Sanberg Journal: Stem Cells Dev Date: 2010-04 Impact factor: 3.272
Authors: Yuji Kaneko; Travis Dailey; Nathan L Weinbren; Jessica Rizzi; Cyrus Tamboli; Julie G Allickson; Nicole Kuzmin-Nichols; Paul R Sanberg; David J Eve; Naoki Tajiri; Cesar V Borlongan Journal: CNS Neurol Disord Drug Targets Date: 2013-05-01 Impact factor: 4.388
Authors: Yanira Franco-Murillo; José Antonio Miranda-Rodríguez; Erika Rendón-Huerta; Luis F Montaño; Gerardo Velázquez Cornejo; Lucila Poblano Gómez; Francisco Javier Valdez-Morales; Ignacio Gonzalez-Sanchez; Marco Cerbón Journal: Reprod Sci Date: 2014-09-06 Impact factor: 3.060