Literature DB >> 14967234

Purification of Toxoplasma gondii oocysts by cesium chloride gradient.

Aurélien Dumètre1, Marie-Laure Dardé.   

Abstract

We describe the use of a cesium chloride (CsCl) gradient as an improvement for the purification of Toxoplasma gondii oocysts from concentrated suspensions. After concentration by sucrose flotation, this technique gives a > 96% recovery of very pure unsporulated or sporulated oocysts, but requires "fresh" oocysts (< or = 10 weeks of age). This material is suitable for biochemical and immunological analyses of environmentally resistant T. gondii oocysts.

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Year:  2004        PMID： 14967234     DOI： 10.1016/j.mimet.2003.11.020

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  15 in total

Review 1.  Interaction forces drive the environmental transmission of pathogenic protozoa.

Authors:  Aurélien Dumètre; Dominique Aubert; Pierre-Henri Puech; Jeanne Hohweyer; Nadine Azas; Isabelle Villena
Journal:  Appl Environ Microbiol       Date:  2011-12-09       Impact factor: 4.792

2.  Determining UV inactivation of Toxoplasma gondii oocysts by using cell culture and a mouse bioassay.

Authors:  Michael W Ware; Swinburne A J Augustine; David O Erisman; Mary Jean See; Larry Wymer; Samuel L Hayes; J P Dubey; Eric N Villegas
Journal:  Appl Environ Microbiol       Date:  2010-06-11       Impact factor: 4.792

3.  Surface properties of Toxoplasma gondii oocysts and surrogate microspheres.

Authors:  Karen Shapiro; John Largier; Jonna A K Mazet; William Bernt; John R Ell; Ann C Melli; Patricia A Conrad
Journal:  Appl Environ Microbiol       Date:  2008-12-05       Impact factor: 4.792

Review 4.  The impact of the waterborne transmission of Toxoplasma gondii and analysis efforts for water detection: an overview and update.

Authors:  Panagiotis Karanis; Hebatalla M Aldeyarbi; Marzieh E Mirhashemi; Khalil M Khalil
Journal:  Environ Sci Pollut Res Int       Date:  2012-09-19       Impact factor: 4.223

5.  Quantitative estimation of the viability of Toxoplasma gondii oocysts in soil.

Authors:  Maud Lélu; Isabelle Villena; Marie-Laure Dardé; Dominique Aubert; Régine Geers; Emilie Dupuis; Francine Marnef; Marie-Lazarine Poulle; Cécile Gotteland; Aurélien Dumètre; Emmanuelle Gilot-Fromont
Journal:  Appl Environ Microbiol       Date:  2012-05-11       Impact factor: 4.792

6.  Obtaining highly purified Toxoplasma gondii oocysts by a discontinuous cesium chloride gradient.

Authors:  Sarah E Staggs; Mary Jean See; J P Dubey; Eric N Villegas
Journal:  J Vis Exp       Date:  2009-11-03       Impact factor: 1.355

7.  Stray dogs as indicators of Toxoplasma gondii distributed in the environment: the first report across an urban-rural gradient in China.

Authors:  Chao Yan; Lin-Lin Fu; Cai-Ling Yue; Ren-Xian Tang; Yi-Sheng Liu; Liang Lv; Na Shi; Ping Zeng; Peng Zhang; Dong-Hui Wang; Dong-Hui Zhou; Xing-Quan Zhu; Kui-Yang Zheng
Journal:  Parasit Vectors       Date:  2012-01-05       Impact factor: 3.876

8.  Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Authors:  Heather M Fritz; Kerry R Buchholz; Xiucui Chen; Blythe Durbin-Johnson; David M Rocke; Patricia A Conrad; John C Boothroyd
Journal:  PLoS One       Date:  2012-02-13       Impact factor: 3.240

9.  Diagnostic value of a Rec-ELISA using Toxoplasma gondii recombinant SporoSAG, BAG1, and GRA1 proteins in murine models infected orally with tissue cysts and oocysts.

Authors:  Mert Döşkaya; Ayşe Caner; Hüseyin Can; Sultan Gülçe İz; Yaprak Gedik; Aysu Değirmenci Döşkaya; Mina Kalantari-Dehaghi; Yüksel Gürüz
Journal:  PLoS One       Date:  2014-09-30       Impact factor: 3.240

10.  Oocyst-Derived Extract of Toxoplasma Gondii Serves as Potent Immunomodulator in a Mouse Model of Birch Pollen Allergy.

Authors:  Angelika Wagner; Irma Schabussova; Mirjana Drinic; Johnnie Akgün; Gerhard Loupal; Michael Kundi; Anja Joachim; Ursula Wiedermann
Journal:  PLoS One       Date:  2016-05-05       Impact factor: 3.240
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