Literature DB >> 1494353

Cloning and DNA sequence analysis of the mercury resistance genes of Streptomyces lividans.

R Sedlmeier1, J Altenbuchner.   

Abstract

A broad-spectrum mercury resistance locus (mer) from a spontaneous chloramphenicol-sensitive (Cms), arginine auxotrophic (Arg-) mutant of Streptomyces lividans 1326 was isolated on a 6 kb DNA fragment by shotgun cloning into the mercury-sensitive derivative S. lividans TK64 using the vector pIJ702. The mer genes form part of a very large amplifiable DNA sequence present in S. lividans 1326. This element was amplified to about 20 copies per chromosome in the Cms Arg- mutant and was missing from strains like S. lividans TK64, cured for the plasmid SLP3. DNA sequence analysis of a 5 kb region encompassing the whole region required for broad-spectrum mercury resistance revealed six open reading frames (ORFs) transcribed in opposite directions from a common intercistronic region. The protein sequences predicted from the two ORFs transcribed in one direction showed a high degree of similarity to mercuric reductase and organomercurial lyase from other gram-negative and gram-positive sources. Few, if any, similarities were found between the predicted polypeptide sequences of the other four ORFs and other known proteins.

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Year:  1992        PMID： 1494353     DOI： 10.1007/bf00279645

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  40 in total

Review 1.  Compilation and analysis of DNA sequences associated with apparent streptomycete promoters.

Authors:  W R Strohl
Journal:  Nucleic Acids Res       Date:  1992-03-11       Impact factor: 16.971

2.  The MerR heavy metal receptor mediates positive activation in a topologically novel transcription complex.

Authors:  T V O'Halloran; B Frantz; M K Shin; D M Ralston; J G Wright
Journal:  Cell       Date:  1989-01-13       Impact factor: 41.582

3.  The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation.

Authors:  J L Marsh; M Erfle; E J Wykes
Journal:  Gene       Date:  1984-12       Impact factor: 3.688

4.  Mercuric reductase: homology to glutathione reductase and lipoamide dehydrogenase. Iodoacetamide alkylation and sequence of the active site peptide.

Authors:  B S Fox; C T Walsh
Journal:  Biochemistry       Date:  1983-08-16       Impact factor: 3.162

5.  An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels.

Authors:  D Tautz; M Renz
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

6.  A simple method for displaying the hydropathic character of a protein.

Authors:  J Kyte; R F Doolittle
Journal:  J Mol Biol       Date:  1982-05-05       Impact factor: 5.469

7.  Cloning and sequencing of the blaZ gene encoding beta-lactamase III, a lipoprotein of Bacillus cereus 569/H.

Authors:  M Hussain; F I Pastor; J O Lampen
Journal:  J Bacteriol       Date:  1987-02       Impact factor: 3.490

8.  Nucleotide sequence of a chromosomal mercury resistance determinant from a Bacillus sp. with broad-spectrum mercury resistance.

Authors:  Y Wang; M Moore; H S Levinson; S Silver; C Walsh; I Mahler
Journal:  J Bacteriol       Date:  1989-01       Impact factor: 3.490

Review 9.  Bacterial resistances to inorganic mercury salts and organomercurials.

Authors:  T K Misra
Journal:  Plasmid       Date:  1992-01       Impact factor: 3.466

10.  The nucleotide sequence of the tyrosinase gene from Streptomyces antibioticus and characterization of the gene product.

Authors:  V Bernan; D Filpula; W Herber; M Bibb; E Katz
Journal:  Gene       Date:  1985       Impact factor: 3.688
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  19 in total

1.  Complete nucleotide sequence of Tn10.

Authors:  R Chalmers; S Sewitz; K Lipkow; P Crellin
Journal:  J Bacteriol       Date:  2000-05       Impact factor: 3.490

2.  Interspecific transfer of Streptomyces giant linear plasmids in sterile amended soil microcosms.

Authors:  J Ravel; E M Wellington; R T Hill
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

3.  Direct measurement of mercury(II) removal from organomercurial lyase (MerB) by tryptophan fluorescence: NmerA domain of coevolved γ-proteobacterial mercuric ion reductase (MerA) is more efficient than MerA catalytic core or glutathione .

Authors:  Baoyu Hong; Rachel Nauss; Ian M Harwood; Susan M Miller
Journal:  Biochemistry       Date:  2010-09-21       Impact factor: 3.162

4.  Regulation of the operon responsible for broad-spectrum mercury resistance in Streptomyces lividans 1326.

Authors:  P Brünker; D Rother; R Sedlmeier; J Klein; R Mattes; J Altenbuchner
Journal:  Mol Gen Genet       Date:  1996-06-12

5.  Zn2+-sensing by the cyanobacterial metallothionein repressor SmtB: different motifs mediate metal-induced protein-DNA dissociation.

Authors:  J S Turner; P D Glands; A C Samson; N J Robinson
Journal:  Nucleic Acids Res       Date:  1996-10-01       Impact factor: 16.971

6.  Isolation and identification of Streptomyces fradiae SU-1 from Thailand and protoplast transformation with the chitinase B Gene from Nocardiopsis prasina OPC-131.

Authors:  Busaya Apichaisataienchote; Josef Altenbuchner; Heinrich Buchenauer
Journal:  Curr Microbiol       Date:  2005-07-05       Impact factor: 2.188

7.  Mercury resistance is encoded by transferable giant linear plasmids in two chesapeake bay Streptomyces strains.

Authors:  J Ravel; H Schrempf; R T Hill
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

8.  The very large amplifiable element AUD2 from Streptomyces lividans 66 has insertion sequence-like repeats at its ends.

Authors:  C Eichenseer; J Altenbuchner
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

9.  Gene cloning, protein characterization, and alteration of product selectivity for the trehalulose hydrolase and trehalulose synthase from "Pseudomonas mesoacidophila" MX-45.

Authors:  Hildegard Watzlawick; Ralf Mattes
Journal:  Appl Environ Microbiol       Date:  2009-09-25       Impact factor: 4.792

10.  The genome of Geobacter bemidjiensis, exemplar for the subsurface clade of Geobacter species that predominate in Fe(III)-reducing subsurface environments.

Authors:  Muktak Aklujkar; Nelson D Young; Dawn Holmes; Milind Chavan; Carla Risso; Hajnalka E Kiss; Cliff S Han; Miriam L Land; Derek R Lovley
Journal:  BMC Genomics       Date:  2010-09-09       Impact factor: 3.969
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