Literature DB >> 1490106

Comparison of the N-glycoloylneuraminic and N-acetylneuraminic acid content of platelets and their precursors using high performance anion exchange chromatography.

T J Budd1, C D Dolman, A M Lawson, W Chai, J Saxton, F W Hemming.   

Abstract

N-Acetylneuraminic acid (Neu5Ac) and N-glycoloylneuraminic acid (Neu5Gc) are distributed widely in nature. Using a Carbopac PA-1 anion exchange column, we have determined the ratios of Neu5Ac and Neu5Gc in hydrolysates of platelets and their precursors: a rat promegakaryoblastic (RPM) cell line and a human megakaryoblastic leukemia cell line (MEG-01). The ratio of Neu5Gc:Neu5Ac in cultured RPM cells is 16:1, whereas in platelet rich plasma and cultured MEG-01 cells it is 1:38 and 1:28, respectively. The nature of these sialic acids from RPM cells was verified using thin layer chromatography and liquid secondary ion mass spectrometry. The relevance of increased Neu5Gc levels in early stages of development is discussed.

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Year:  1992        PMID： 1490106     DOI： 10.1007/bf00731139

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  17 in total

1.  High-pressure liquid chromatography of sialic acids on a pellicular resin anion-exchange column with pulsed amperometric detection: a comparison with six other systems.

Authors:  A E Manzi; S Diaz; A Varki
Journal:  Anal Biochem       Date:  1990-07       Impact factor: 3.365

2.  Sialic Acid: its importance to platelet function in health and disease.

Authors:  M Crook
Journal:  Platelets       Date:  1991       Impact factor: 3.862

3.  High-sensitivity structural analyses of oligosaccharide probes (neoglycolipids) by liquid-secondary-ion mass spectrometry.

Authors:  A M Lawson; W G Chai; G C Cashmore; M S Stoll; E F Hounsell; T Feizi
Journal:  Carbohydr Res       Date:  1990-04-25       Impact factor: 2.104

4.  Isolation of platelets from laboratory animals.

Authors:  J L Catalfamo; W J Dodds
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

5.  Abnormal intrapulmonary platelet production: a possible cause of vascular and lung disease.

Authors:  J F Martin; D N Slater; E A Trowbridge
Journal:  Lancet       Date:  1983-04-09       Impact factor: 79.321

6.  The morphological and biochemical characterization of a line of rat promegakaryoblasts.

Authors:  R Weinstein; M B Stemerman; D E MacIntyre; H N Steinberg; T Maciag
Journal:  Blood       Date:  1981-07       Impact factor: 22.113

7.  Detection of CMP-N-acetylneuraminic acid hydroxylase activity in fractionated mouse liver.

Authors:  L Shaw; R Schauer
Journal:  Biochem J       Date:  1989-10-15       Impact factor: 3.857

8.  Sialic acid in mature megakaryocytes: detection by wheat germ agglutinin.

Authors:  P K Schick; W G Filmyer
Journal:  Blood       Date:  1985-05       Impact factor: 22.113

9.  Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome.

Authors:  M Ogura; Y Morishima; R Ohno; Y Kato; N Hirabayashi; H Nagura; H Saito
Journal:  Blood       Date:  1985-12       Impact factor: 22.113

10.  Quantitative determination of N-glycolylneuraminic acid expression in human cancerous tissues and avian lymphoma cell lines as a tumor-associated sialic acid by gas chromatography-mass spectrometry.

Authors:  T Kawai; A Kato; H Higashi; S Kato; M Naiki
Journal:  Cancer Res       Date:  1991-02-15       Impact factor: 12.701
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  1 in total

1.  A serotonin-induced N-glycan switch regulates platelet aggregation.

Authors:  Charles P Mercado; Maritza V Quintero; Yicong Li; Preeti Singh; Alicia K Byrd; Krajang Talabnin; Mayumi Ishihara; Parastoo Azadi; Nancy J Rusch; Balagurunathan Kuberan; Luc Maroteaux; Fusun Kilic
Journal:  Sci Rep       Date:  2013-09-30       Impact factor: 4.379
  1 in total

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