Literature DB >> 1481278

Has negative staining still a place in biomacromolecular electron microscopy?

A Bremer1, C Henn, A Engel, W Baumeister, U Aebi.   

Abstract

Transmission electron microscopy of proteins has provided molecular- and in a few cases near-atomic-resolution structural information. In this review, we critically evaluate the potential and the limitations in obtaining molecular resolution, particularly with negatively stained specimens, and put these into perspective with cryomicroscopy of unstained frozen-hydrated and sugar-embedded preparations.

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Year:  1992        PMID： 1481278     DOI： 10.1016/0304-3991(92)90008-8

Source DB:  PubMed          Journal:  Ultramicroscopy        ISSN： 0304-3991            Impact factor:   2.689


  17 in total

1.  Tapping-mode atomic force microscopy produces faithful high-resolution images of protein surfaces.

Authors:  C Möller; M Allen; V Elings; A Engel; D J Müller
Journal:  Biophys J       Date:  1999-08       Impact factor: 4.033

2.  The height of biomolecules measured with the atomic force microscope depends on electrostatic interactions.

Authors:  D J Müller; A Engel
Journal:  Biophys J       Date:  1997-09       Impact factor: 4.033

3.  3D imaging and quantitative analysis of small solubilized membrane proteins and their complexes by transmission electron microscopy.

Authors:  Ardeschir Vahedi-Faridi; Beata Jastrzebska; Krzysztof Palczewski; Andreas Engel
Journal:  Microscopy (Oxf)       Date:  2012-12-23       Impact factor: 1.571

4.  Studying protein-reconstituted proteoliposome fusion with content indicators in vitro.

Authors:  Jiajie Diao; Minglei Zhao; Yunxiang Zhang; Minjoung Kyoung; Axel T Brunger
Journal:  Bioessays       Date:  2013-04-29       Impact factor: 4.345

5.  Structure of intracellular mature vaccinia virus observed by cryoelectron microscopy.

Authors:  J Dubochet; M Adrian; K Richter; J Garces; R Wittek
Journal:  J Virol       Date:  1994-03       Impact factor: 5.103

Review 6.  Lipid environment of membrane proteins in cryo-EM based structural analysis.

Authors:  Kazuhiro Mio; Chikara Sato
Journal:  Biophys Rev       Date:  2017-12-18

Review 7.  Optimized negative-staining electron microscopy for lipoprotein studies.

Authors:  Lei Zhang; Huimin Tong; Mark Garewal; Gang Ren
Journal:  Biochim Biophys Acta       Date:  2012-09-29

8.  Structure-function relationships of the outer membrane translocon Wza investigated by cryo-electron microscopy and mutagenesis.

Authors:  Robert C Ford; Anne L Brunkan-LaMontagne; Richard F Collins; Bradley R Clarke; Robert Harris; James H Naismith; Chris Whitfield
Journal:  J Struct Biol       Date:  2009-02-21       Impact factor: 2.867

9.  Electron microscopy localization and characterization of functionalized composite organic-inorganic SERS nanoparticles on leukemia cells.

Authors:  Ai Leen Koh; Catherine M Shachaf; Sailaja Elchuri; Garry P Nolan; Robert Sinclair
Journal:  Ultramicroscopy       Date:  2008-10-02       Impact factor: 2.689

10.  Plasticity of intermediate filament subunits.

Authors:  Robert Kirmse; Zhao Qin; Carl M Weinert; Andreas Hoenger; Andrea Hoenger; Markus J Buehler; Laurent Kreplak
Journal:  PLoS One       Date:  2010-08-12       Impact factor: 3.240
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