Literature DB >> 14766869

Development of a serum-based Taqman real-time PCR assay for diagnosis of invasive aspergillosis.

Svetlana Challier1, Stéphanie Boyer, Eric Abachin, Patrick Berche.   

Abstract

We compared an Aspergillus fumigatus-specific Taqman real-time PCR assay for the diagnosis of invasive aspergillosis (IA) with the enzyme-linked immunosorbent assay Platelia Aspergillus method. Patients with proven or probable IA had positive results with at least one of the two methods. The PCR method seems to be more specific, and a combination of the two should improve the diagnosis of IA.

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Year:  2004        PMID： 14766869      PMCID： PMC344496          DOI： 10.1128/JCM.42.2.844-846.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system.

Authors:  J Loeffler; N Henke; H Hebart; D Schmidt; L Hagmeyer; U Schumacher; H Einsele
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Screening for Aspergillus spp. using polymerase chain reaction of whole blood samples from patients with haematological malignancies.

Authors:  C Lass-Flörl; J Aigner; E Gunsilius; A Petzer; D Nachbaur; G Gastl; H Einsele; J Löffler; M P Dierich; R Würzner
Journal:  Br J Haematol       Date:  2001-04       Impact factor: 6.998

3.  Early detection of aspergillus infection after allogeneic stem cell transplantation by polymerase chain reaction screening.

Authors:  H Hebart; J Löffler; C Meisner; F Serey; D Schmidt; A Böhme; H Martin; A Engel; D Bunje; W V Kern; U Schumacher; L Kanz; H Einsele
Journal:  J Infect Dis       Date:  2000-05-15       Impact factor: 5.226

4.  Development of two real-time quantitative TaqMan PCR assays to detect circulating Aspergillus fumigatus DNA in serum.

Authors:  C Costa; D Vidaud; M Olivi; E Bart-Delabesse; M Vidaud; S Bretagne
Journal:  J Microbiol Methods       Date:  2001-04       Impact factor: 2.363

Review 5.  Invasive aspergillosis.

Authors:  B Dupont; M Richardson; P E Verweij; J F Meis
Journal:  Med Mycol       Date:  2000       Impact factor: 4.076

6.  Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: an international consensus.

Authors:  S Ascioglu; J H Rex; B de Pauw; J E Bennett; J Bille; F Crokaert; D W Denning; J P Donnelly; J E Edwards; Z Erjavec; D Fiere; O Lortholary; J Maertens; J F Meis; T F Patterson; J Ritter; D Selleslag; P M Shah; D A Stevens; T J Walsh
Journal:  Clin Infect Dis       Date:  2001-11-26       Impact factor: 9.079

7.  Specific detection of Aspergillus species in blood and bronchoalveolar lavage samples of immunocompromised patients by two-step PCR.

Authors:  H Skladny; D Buchheidt; C Baust; F Krieg-Schneider; W Seifarth; C Leib-Mösch; R Hehlmann
Journal:  J Clin Microbiol       Date:  1999-12       Impact factor: 5.948

8.  Value of antigen detection using an enzyme immunoassay in the diagnosis and prediction of invasive aspergillosis in two adult and pediatric hematology units during a 4-year prospective study.

Authors:  A Sulahian; F Boutboul; P Ribaud; T Leblanc; C Lacroix; F Derouin
Journal:  Cancer       Date:  2001-01-15       Impact factor: 6.860

9.  Comparison between plasma and whole blood specimens for detection of Aspergillus DNA by PCR.

Authors:  J Loeffler; H Hebart; U Brauchle; U Schumacher; H Einsele
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

10.  Autopsy-controlled prospective evaluation of serial screening for circulating galactomannan by a sandwich enzyme-linked immunosorbent assay for hematological patients at risk for invasive Aspergillosis.

Authors:  J Maertens; J Verhaegen; H Demuynck; P Brock; G Verhoef; P Vandenberghe; J Van Eldere; L Verbist; M Boogaerts
Journal:  J Clin Microbiol       Date:  1999-10       Impact factor: 5.948
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  29 in total

1.  Use of real-time PCR to process the first galactomannan-positive serum sample in diagnosing invasive aspergillosis.

Authors:  Laurence Millon; Renaud Piarroux; Eric Deconinck; Claude-Eric Bulabois; Frédéric Grenouillet; Pierre Rohrlich; Jean-Marc Costa; Stéphane Bretagne
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

2.  Development of a nested qualitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens.

Authors:  Catriona Halliday; Qi Xuan Wu; Gregory James; Tania Sorrell
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

Review 3.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

4.  Development of novel real-time PCR assays for detection and differentiation of eleven medically important Aspergillus and Candida species in clinical specimens.

Authors:  Claudia Schabereiter-Gurtner; Brigitte Selitsch; Manfred L Rotter; Alexander M Hirschl; Birgit Willinger
Journal:  J Clin Microbiol       Date:  2007-01-24       Impact factor: 5.948

5.  Quantitative Real-Time PCR and Platelia Galactomannan Assay for the Diagnosis of Invasive Pulmonary Aspergillosis: Bronchoalveolar Lavage Fluid Performs Better Than Serum in Non-neutropaenic Patients.

Authors:  Shuzhen Zhang; Sibu Wang; Zhe Wan; Chengli Que; Ruoyu Li; Jin Yu
Journal:  Mycopathologia       Date:  2016-06-07       Impact factor: 2.574

6.  Development of a DNA microarray for detection and identification of fungal pathogens involved in invasive mycoses.

Authors:  Dirk M Leinberger; Ulrike Schumacher; Ingo B Autenrieth; Till T Bachmann
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

7.  Investigating the presence of fungal agents in febrile neutropenic patients using different microbiological, serological, and molecular methods.

Authors:  Ahu Sönmez; Fahriye Eksi; Mustafa Pehlivan; Handan Haydaroglu Sahin
Journal:  Bosn J Basic Med Sci       Date:  2015-07-27       Impact factor: 3.363

Review 8.  Molecular and nonmolecular diagnostic methods for invasive fungal infections.

Authors:  Marios Arvanitis; Theodora Anagnostou; Beth Burgwyn Fuchs; Angela M Caliendo; Eleftherios Mylonakis
Journal:  Clin Microbiol Rev       Date:  2014-07       Impact factor: 26.132

9.  Multicenter comparison of serum and whole-blood specimens for detection of Aspergillus DNA in high-risk hematological patients.

Authors:  Jan Springer; C O Morton; Michael Perry; Werner J Heinz; Melinda Paholcsek; Mona Alzheimer; T R Rogers; Rosemary A Barnes; Hermann Einsele; Juergen Loeffler; P Lewis White
Journal:  J Clin Microbiol       Date:  2013-02-20       Impact factor: 5.948

Review 10.  PCR-based diagnosis of human fungal infections.

Authors:  Prasanna D Khot; David N Fredricks
Journal:  Expert Rev Anti Infect Ther       Date:  2009-12       Impact factor: 5.091
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