Literature DB >> 14763758

Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thin-layer chromatography.

Thomas Larsen1, Jørgen Axelsen, Helle Weber Ravn.   

Abstract

A new and simplified method for extraction of ergosterol (ergosta-5,7,22-trien-3beta-ol) from fungi in soil and litter was developed using pre-soaking extraction and paraffin oil for recovery. Recoveries of ergosterol were in the range of 94-100% depending on the solvent to oil ratio. Extraction efficiencies equal to heat-assisted extraction treatments were obtained with pre-soaking extraction. Ergosterol was detected with thin-layer chromatography (TLC) using fluorodensitometry with a quantification limit of 8 ng. Using visual evaluation of images of TLC plates photographed in UV-light the quantification limit was 16 ng.

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Year:  2004        PMID： 14763758     DOI： 10.1016/j.chroma.2003.10.128

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  5 in total

1.  Mesoionic compounds with antifungal activity against Fusarium verticillioides.

Authors:  Rojane de Oliveira Paiva; Lucimar Ferreira Kneipp; Camilla Moretto dos Reis; Aurea Echevarria
Journal:  BMC Microbiol       Date:  2015-02-04       Impact factor: 3.605

2.  1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa.

Authors:  Marcela Queiroz Granato; Diego de Souza Gonçalves; Sergio Henrique Seabra; Malachy McCann; Michael Devereux; André Luis Souza Dos Santos; Lucimar Ferreira Kneipp
Journal:  Front Microbiol       Date:  2017-01-30       Impact factor: 5.640

3.  HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi.

Authors:  Vanila F Palmeira; Daniela S Alviano; Lys A Braga-Silva; Fátima R V Goulart; Marcela Q Granato; Sonia Rozental; Celuta S Alviano; André L S Santos; Lucimar F Kneipp
Journal:  Front Microbiol       Date:  2017-05-19       Impact factor: 5.640

4.  Repositioning Lopinavir, an HIV Protease Inhibitor, as a Promising Antifungal Drug: Lessons Learned from Candida albicans-In Silico, In Vitro and In Vivo Approaches.

Authors:  André L S Santos; Lys A Braga-Silva; Diego S Gonçalves; Lívia S Ramos; Simone S C Oliveira; Lucieri O P Souza; Vanessa S Oliveira; Roberto D Lins; Marcia R Pinto; Julian E Muñoz; Carlos P Taborda; Marta H Branquinha
Journal:  J Fungi (Basel)       Date:  2021-05-28

5.  Matrix Solid-Phase Dispersion Extraction and Quantification of Alpinetin in Amomum Seed using Validated HPLC and HPTLC Methods.

Authors:  M Singh; Y T Kamal; M A Khan; Rabea Parveen; S H Ansari; S Ahmad
Journal:  Indian J Pharm Sci       Date:  2015 Jan-Feb       Impact factor: 0.975
  5 in total

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