AIM: To explore the effect of antisense oligodeoxynucleotide (As-ODN) of telomerase RNA on telomerase activity and cell apoptosis in human colon cancer. METHODS: As-ODN was transfected into SW480 cells by liposomal transfection reagent. Telomerase activity of SW480 cells was examined by telomeric repeat amplification protocol (TRAP) and enzyme-linked immunosorbent assay (ELISA). Apoptosis was analyzed by morphology and flow cytometry. RESULTS: The telomerase activity in SW480 cells transfected with 1.0 micromol/L of As-ODN for 2-5 days, was significantly decreased in a time-dependent manner, and the cells underwent apoptosis. The missense ODN (Ms-ODN) and the control group transfected with SW480 cells did not show these changes. CONCLUSION: As-ODN can specifically inhibit the telomerase activity of SW480 cells and induce apoptosis.
AIM: To explore the effect of antisense oligodeoxynucleotide (As-ODN) of telomerase RNA on telomerase activity and cell apoptosis in humancolon cancer. METHODS:As-ODN was transfected into SW480 cells by liposomal transfection reagent. Telomerase activity of SW480 cells was examined by telomeric repeat amplification protocol (TRAP) and enzyme-linked immunosorbent assay (ELISA). Apoptosis was analyzed by morphology and flow cytometry. RESULTS: The telomerase activity in SW480 cells transfected with 1.0 micromol/L of As-ODN for 2-5 days, was significantly decreased in a time-dependent manner, and the cells underwent apoptosis. The missense ODN (Ms-ODN) and the control group transfected with SW480 cells did not show these changes. CONCLUSION:As-ODN can specifically inhibit the telomerase activity of SW480 cells and induce apoptosis.
Authors: D M Kushner; J M Paranjape; B Bandyopadhyay; H Cramer; D W Leaman; A W Kennedy; R H Silverman; J K Cowell Journal: Gynecol Oncol Date: 2000-02 Impact factor: 5.482
Authors: B Herbert; A E Pitts; S I Baker; S E Hamilton; W E Wright; J W Shay; D R Corey Journal: Proc Natl Acad Sci U S A Date: 1999-12-07 Impact factor: 11.205
Authors: J S Dome; S Chung; T Bergemann; C B Umbricht; M Saji; L A Carey; P E Grundy; E J Perlman; N E Breslow; S Sukumar Journal: Cancer Res Date: 1999-09-01 Impact factor: 12.701