Literature DB >> 14749530

The binding of ethidium bromide with DNA: interaction with single- and double-stranded structures.

P O Vardevanyan1, A P Antonyan, M A Parsadanyan, H G Davtyan, A T Karapetyan.   

Abstract

The pH-induced helix-coil transition of DNA and its complexes with EtBr is carried out at acidic pH in a wide interval of change of concentration ratio of EtBr/DNA. The binding isotherms of EtBr on double and single-stranded DNA at pH = 7.0 and pH = 3.0 (t = 25(o)C) are obtained by absorption and fluorimetric methods. Binding constants (K) and number of bases (n), corresponding to one binding site were determined. Non fluorescent "strong" complex with ds-DNA at pH = 7.0 and t = 25(o)C as well as "strong" and "weak" complexes with ss-DNA at pH = 3.0 and t = 25(o)C are revealed.

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Year:  2003        PMID： 14749530     DOI： 10.1038/emm.2003.68

Source DB:  PubMed          Journal:  Exp Mol Med        ISSN： 1226-3613            Impact factor:   8.718


  16 in total

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