BACKGROUND: Cleavage stage embryos as well as postimplantation embryos have been studied extensively over the years. However, our knowledge with respect to the chromosomal constitution of human embryos at the blastocyst stage is still rudimentary. METHODS: In the present paper, a large series of human blastocysts was examined by means of fluorescent in situ hybridization (FISH). RESULTS: It was found that only one in four blastocysts (25%) displayed a normal chromosomal pattern. We defined a group of blastocysts (26%) displaying a simple mosaic chromosome pattern (different cell lines resulting from one chromosomal error), an about equally large group of blastocysts (31%) displaying a complex mosaic chromosome pattern, and a smaller group of blastocysts (11%) showing a chaotic chromosome distribution pattern. Six per cent of all blastocysts analysed could not be assigned one of the previously mentioned chromosomal patterns. CONCLUSION: Anaphase lagging appeared to be the major mechanism through which human embryos acquire a mosaic chromosome pattern during preimplantation development to the blastocyst stage.
BACKGROUND: Cleavage stage embryos as well as postimplantation embryos have been studied extensively over the years. However, our knowledge with respect to the chromosomal constitution of human embryos at the blastocyst stage is still rudimentary. METHODS: In the present paper, a large series of humanblastocysts was examined by means of fluorescent in situ hybridization (FISH). RESULTS: It was found that only one in four blastocysts (25%) displayed a normal chromosomal pattern. We defined a group of blastocysts (26%) displaying a simple mosaic chromosome pattern (different cell lines resulting from one chromosomal error), an about equally large group of blastocysts (31%) displaying a complex mosaic chromosome pattern, and a smaller group of blastocysts (11%) showing a chaotic chromosome distribution pattern. Six per cent of all blastocysts analysed could not be assigned one of the previously mentioned chromosomal patterns. CONCLUSION: Anaphase lagging appeared to be the major mechanism through which human embryos acquire a mosaic chromosome pattern during preimplantation development to the blastocyst stage.
Authors: D Ioannou; K G L Fonseka; E J Meershoek; A R Thornhill; A Abogrein; M Ellis; D K Griffin Journal: Chromosome Res Date: 2012-06-29 Impact factor: 5.239
Authors: Sandra Zamora; Ana Clavero; M Carmen Gonzalvo; Juan de Dios Luna Del Castillo; Jose Antonio Roldán-Nofuentes; Juan Mozas; Jose Antonio Castilla Journal: J Assist Reprod Genet Date: 2011-06-29 Impact factor: 3.412
Authors: Oleksii O Barash; Kristen A Ivani; Susan P Willman; Evan M Rosenbluth; Deborah S Wachs; Mary D Hinckley; Sara Pittenger Reid; Louis N Weckstein Journal: J Assist Reprod Genet Date: 2017-05-30 Impact factor: 3.412
Authors: D S Johnson; C Cinnioglu; R Ross; A Filby; G Gemelos; M Hill; A Ryan; D Smotrich; M Rabinowitz; M J Murray Journal: Mol Hum Reprod Date: 2010-07-19 Impact factor: 4.025