BACKGROUND: Volatile anesthetics disappear from an organism after the end of anesthesia. Whether changes of protein expression persist in the brain for a longer period is not known. This study investigates the question of whether the expression of proteins is altered in the rat brain after the end of desflurane anesthesia. METHODS: Three groups (n = 12 each) of rats were anesthetized with 5.7% desflurane in air for 3 h. Brains were removed directly after anesthesia, 24 h after anesthesia, or 72 h after anesthesia. Two additional groups (n = 12 each) served as naive conscious controls, in which the brains were removed without previous anesthesia 3 or 72 h after the start of the experiment. Cytosolic proteins were isolated. A proteome-wide study was performed, based on two-dimensional gel electrophoresis and mass spectrometry. RESULTS: Compared with conscious controls, significant (P < 0.05) increase/decrease was found: 3 h of anesthesia, 5/2 proteins; 24 h after anesthesia, 13/1 proteins; 72 h after anesthesia, 6/4 proteins. The overall changes in protein expression as quantified by the induction factor ranged from -1.67 (decrease to 60%) to 1.79 (increase by 79%) compared with the controls (100%). Some of these regulated proteins play a role in vesicle transport and metabolism. CONCLUSION: Desflurane anesthesia produces changes in cytosolic protein expression up to 72 h after anesthesia in the rat brain, indicating yet unknown persisting effects.
BACKGROUND: Volatile anesthetics disappear from an organism after the end of anesthesia. Whether changes of protein expression persist in the brain for a longer period is not known. This study investigates the question of whether the expression of proteins is altered in the rat brain after the end of desflurane anesthesia. METHODS: Three groups (n = 12 each) of rats were anesthetized with 5.7% desflurane in air for 3 h. Brains were removed directly after anesthesia, 24 h after anesthesia, or 72 h after anesthesia. Two additional groups (n = 12 each) served as naive conscious controls, in which the brains were removed without previous anesthesia 3 or 72 h after the start of the experiment. Cytosolic proteins were isolated. A proteome-wide study was performed, based on two-dimensional gel electrophoresis and mass spectrometry. RESULTS: Compared with conscious controls, significant (P < 0.05) increase/decrease was found: 3 h of anesthesia, 5/2 proteins; 24 h after anesthesia, 13/1 proteins; 72 h after anesthesia, 6/4 proteins. The overall changes in protein expression as quantified by the induction factor ranged from -1.67 (decrease to 60%) to 1.79 (increase by 79%) compared with the controls (100%). Some of these regulated proteins play a role in vesicle transport and metabolism. CONCLUSION:Desflurane anesthesia produces changes in cytosolic protein expression up to 72 h after anesthesia in the rat brain, indicating yet unknown persisting effects.
Authors: Junxia X Tang; Feras Mardini; Breanna M Caltagarone; Sean T Garrity; Rosie Q Li; Shannon L Bianchi; Olubusola Gomes; Frank M Laferla; Roderic G Eckenhoff; Maryellen F Eckenhoff Journal: Alzheimers Dement Date: 2011-07-13 Impact factor: 21.566
Authors: Niccolò Terrando; Marek Brzezinski; Vincent Degos; Lars I Eriksson; Joel H Kramer; Jacqueline M Leung; Bruce L Miller; William W Seeley; Susana Vacas; Michael W Weiner; Kristine Yaffe; William L Young; Zhongcong Xie; Mervyn Maze Journal: Mayo Clin Proc Date: 2011-09 Impact factor: 7.616
Authors: Travis J A Craddock; Marc St George; Holly Freedman; Khaled H Barakat; Sambasivarao Damaraju; Stuart Hameroff; Jack A Tuszynski Journal: PLoS One Date: 2012-06-25 Impact factor: 3.240