Literature DB >> 14719830

A simple protocol for isolation of fungal DNA.

Sachin R Tendulkar1, Archna Gupta, Bharat B Chattoo.   

Abstract

Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phytopathogen in different regions, and for a quick screening of M. grisea transformants.

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Year:  2003        PMID： 14719830     DOI： 10.1023/b:bile.0000003990.27624.04

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  8 in total

1.  The PKS4 gene of Fusarium graminearum is essential for zearalenone production.

Authors:  Erik Lysøe; Sonja S Klemsdal; Karen R Bone; Rasmus J N Frandsen; Thomas Johansen; Ulf Thrane; Henriette Giese
Journal:  Appl Environ Microbiol       Date:  2006-06       Impact factor: 4.792

2.  A rapid PCR-based approach for molecular identification of filamentous fungi.

Authors:  Yuanyuan Chen; Bernard A Prior; Guiyang Shi; Zhengxiang Wang
Journal:  J Microbiol       Date:  2011-09-02       Impact factor: 3.422

3.  Skp1, a component of E3 ubiquitin ligase, is necessary for growth, sporulation, development and pathogenicity in rice blast fungus (Magnaporthe oryzae).

Authors:  Chandra Prakash; Johannes Manjrekar; Bharat B Chattoo
Journal:  Mol Plant Pathol       Date:  2016-01-06       Impact factor: 5.663

4.  Rapid sex identification method of spinach (Spinacia oleracea L.) in the vegetative stage using loop-mediated isothermal amplification.

Authors:  Naoko Fujita; Yu Ayukawa; Mitsutoshi Fuke; Tohru Teraoka; Kyoko Watanabe; Tsutomu Arie; Ken Komatsu
Journal:  Planta       Date:  2016-11-12       Impact factor: 4.116

5.  Petunia floral defensins with unique prodomains as novel candidates for development of fusarium wilt resistance in transgenic banana plants.

Authors:  Siddhesh B Ghag; Upendra K Singh Shekhawat; Thumballi R Ganapathi
Journal:  PLoS One       Date:  2012-06-22       Impact factor: 3.240

6.  Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi.

Authors:  Rasmus J N Frandsen; Jens A Andersson; Matilde B Kristensen; Henriette Giese
Journal:  BMC Mol Biol       Date:  2008-08-01       Impact factor: 2.946

7.  Isolation and characterization of Aspergillus niger NBC001 underlying suppression against Heterodera glycines.

Authors:  Na Jin; Shi-Ming Liu; Huan Peng; Wen-Kun Huang; Ling-An Kong; Yu-Huan Wu; Yong-Pan Chen; Feng-Yong Ge; Heng Jian; De-Liang Peng
Journal:  Sci Rep       Date:  2019-01-24       Impact factor: 4.379

8.  Unravelling the microbiome of eggs of the endangered sea turtle Eretmochelys imbricata identifies bacteria with activity against the emerging pathogen Fusarium falciforme.

Authors:  Jullie M Sarmiento-Ramírez; Menno van der Voort; Jos M Raaijmakers; Javier Diéguez-Uribeondo
Journal:  PLoS One       Date:  2014-04-17       Impact factor: 3.240
  8 in total

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