Literature DB >> 14715800

Automated procedure for improving the RNA isolation step in viral load testing for human immunodeficiency virus.

Min Xu1, Yung Chan, Steven H Fischer, Alan T Remaley.   

Abstract

An automated RNA isolation procedure on the Qiagen BioRobot is described for performing viral load tests for human immunodeficiency virus (HIV) with the Amplicor HIV type 1 test. The new procedure improves the precision of the assay and requires significantly less labor than the presently used manual RNA isolation procedure.

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Year:  2004        PMID： 14715800      PMCID： PMC321698          DOI： 10.1128/JCM.42.1.439-440.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  4 in total

1.  Evaluation of automated nucleic acid extraction devices for application in HCV NAT.

Authors:  J M Jongerius; M Bovenhorst; C L van der Poel; J A van Hilten; A C Kroes; J A van der Does; E F van Leeuwen; R Schuurman
Journal:  Transfusion       Date:  2000-07       Impact factor: 3.157

2.  Improvement of AMPLICOR human immunodeficiency virus type 1 viral load test (version 1.5) by addition of a coprecipitant during the RNA isolation step.

Authors:  Min Xu; Yung Chan; Steven H Fischer; Alan T Remaley
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

3.  COBAS AMPLICOR: fully automated RNA and DNA amplification and detection system for routine diagnostic PCR.

Authors:  N DiDomenico; H Link; R Knobel; T Caratsch; W Weschler; Z G Loewy; M Rosenstraus
Journal:  Clin Chem       Date:  1996-12       Impact factor: 8.327

4.  Efficiencies of four versions of the AMPLICOR HIV-1 MONITOR test for quantification of different subtypes of human immunodeficiency virus type 1.

Authors:  K Triques; J Coste; J L Perret; C Segarra; E Mpoudi; J Reynes; E Delaporte; A Butcher; K Dreyer; S Herman; J Spadoro; M Peeters
Journal:  J Clin Microbiol       Date:  1999-01       Impact factor: 5.948
  4 in total

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