Literature DB >> 14711696

Simple and reliable method to precipitate proteins from bacterial culture supernatant.

Randolph B Caldwell1, Claus T Lattemann.   

Abstract

A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional protocols.

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Year:  2004        PMID： 14711696      PMCID： PMC321231          DOI： 10.1128/AEM.70.1.610-612.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  Precipitation of dilute chromatographic samples (ng/ml) containing interfering substances for SDS-PAGE.

Authors:  R M Aguilar; J J Bustamante; P G Hernandez; A O Martinez; L S Haro
Journal:  Anal Biochem       Date:  1999-02-15       Impact factor: 3.365

2.  Genes encoding putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2 are required for bacterial virulence and proliferation in macrophages.

Authors:  M Hensel; J E Shea; S R Waterman; R Mundy; T Nikolaus; G Banks; A Vazquez-Torres; C Gleeson; F C Fang; D W Holden
Journal:  Mol Microbiol       Date:  1998-10       Impact factor: 3.501

Review 3.  Type III protein secretion systems in bacterial pathogens of animals and plants.

Authors:  C J Hueck
Journal:  Microbiol Mol Biol Rev       Date:  1998-06       Impact factor: 11.056

4.  The mechanism of 2,2,2-trichloroacetic acid-induced protein precipitation.

Authors:  T Sivaraman; T K Kumar; G Jayaraman; C Yu
Journal:  J Protein Chem       Date:  1997-05

5.  Trichloroacetic acid precipitation by ultracentrifugation to concentrate dilute protein in viscous solution.

Authors:  B J Hwang; G Chu
Journal:  Biotechniques       Date:  1996-06       Impact factor: 1.993

6.  Secretion of Yop proteins by Yersiniae.

Authors:  T Michiels; P Wattiau; R Brasseur; J M Ruysschaert; G Cornelis
Journal:  Infect Immun       Date:  1990-09       Impact factor: 3.441

7.  Yersinia-hsp60-reactive T cells are efficiently stimulated by peptides of 12 and 13 amino acid residues in a MHC class II (I-Ab)-restricted manner.

Authors:  A Noll; I B Autenrieth
Journal:  Clin Exp Immunol       Date:  1996-08       Impact factor: 4.330

8.  Urinary protein as measured with a pyrogallol red-molybdate complex, manually and in a Hitachi 726 automated analyzer.

Authors:  N Watanabe; S Kamei; A Ohkubo; M Yamanaka; S Ohsawa; K Makino; K Tokuda
Journal:  Clin Chem       Date:  1986-08       Impact factor: 8.327

9.  Identification of a virulence locus encoding a second type III secretion system in Salmonella typhimurium.

Authors:  J E Shea; M Hensel; C Gleeson; D W Holden
Journal:  Proc Natl Acad Sci U S A       Date:  1996-03-19       Impact factor: 11.205

10.  Precipitation of Hevea brasiliensis latex proteins with trichloroacetic acid and phosphotungstic acid in preparation for the Lowry protein assay.

Authors:  H Y Yeang; F Yusof; L Abdullah
Journal:  Anal Biochem       Date:  1995-03-20       Impact factor: 3.365
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  16 in total

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Authors:  Yan Chen; Guangping Li; Yanxia Liu; Victoria P Werth; Kevin Jon Williams; Ming-Lin Liu
Journal:  J Cell Physiol       Date:  2016-03-09       Impact factor: 6.384

2.  A homolog of Bacillus subtilis trigger factor in Listeria monocytogenes is involved in stress tolerance and bacterial virulence.

Authors:  Armelle Bigot; Eleonore Botton; Iharilalao Dubail; Alain Charbit
Journal:  Appl Environ Microbiol       Date:  2006-10       Impact factor: 4.792

3.  Modulation of an outer membrane protease contributes to the virulence defect of Shigella flexneri strains carrying a mutation in the virK locus.

Authors:  Helen J Wing; Seth R Goldman; Shabeen Ally; Marcia B Goldberg
Journal:  Infect Immun       Date:  2005-02       Impact factor: 3.441

4.  Proteomic analysis of the Vibrio cholerae type II secretome reveals new proteins, including three related serine proteases.

Authors:  Aleksandra E Sikora; Ryszard A Zielke; Daniel A Lawrence; Philip C Andrews; Maria Sandkvist
Journal:  J Biol Chem       Date:  2011-03-08       Impact factor: 5.157

5.  Characterization of a hemophore-like protein from Porphyromonas gingivalis.

Authors:  Jin-Long Gao; Ky-Anh Nguyen; Neil Hunter
Journal:  J Biol Chem       Date:  2010-10-12       Impact factor: 5.157

6.  Membrane disruption by antimicrobial fatty acids releases low-molecular-weight proteins from Staphylococcus aureus.

Authors:  Joshua B Parsons; Jiangwei Yao; Matthew W Frank; Pamela Jackson; Charles O Rock
Journal:  J Bacteriol       Date:  2012-07-27       Impact factor: 3.490

7.  Search for hydrophilic marine fungal metabolites: a rational approach for their production and extraction in a bioactivity screening context.

Authors:  Carine Le Ker; Karina-Ethel Petit; Jean-François Biard; Joël Fleurence
Journal:  Mar Drugs       Date:  2011-01-10       Impact factor: 5.118

8.  Growth of Acidithiobacillus Ferrooxidans ATCC 23270 in Thiosulfate Under Oxygen-Limiting Conditions Generates Extracellular Sulfur Globules by Means of a Secreted Tetrathionate Hydrolase.

Authors:  Simón Beard; Alberto Paradela; Juan P Albar; Carlos A Jerez
Journal:  Front Microbiol       Date:  2011-04-18       Impact factor: 5.640

9.  Evaluation of a Salmonella vectored vaccine expressing Mycobacterium avium subsp. paratuberculosis antigens against challenge in a goat model.

Authors:  Syed M Faisal; Falong Yan; Tsai-Tzu Chen; Nicodemus M Useh; Shanguang Guo; Weiwei Yan; Shih-Jon Wang; Amy L Glaser; Sean P McDonough; Bhupinder Singh; Yung-Fu Chang
Journal:  PLoS One       Date:  2013-08-09       Impact factor: 3.240

10.  Proteomic analysis of the secretome of Leishmania donovani.

Authors:  J Maxwell Silverman; Simon K Chan; Dale P Robinson; Dennis M Dwyer; Devki Nandan; Leonard J Foster; Neil E Reiner
Journal:  Genome Biol       Date:  2008-02-18       Impact factor: 13.583
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