Literature DB >> 14704359

Ligase-mediated construction of branched DNA strands: a novel DNA joining activity catalyzed by T4 DNA ligase.

Maritha Mendel-Hartvig1, Anil Kumar, Ulf Landegren.   

Abstract

Branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating DNA also presents interesting opportunities in biotechnological applications. We describe here how T4 DNA ligase can be used for efficient construction of DNA molecules having one 5' end but two distinct 3' ends that extend from the 2' and 3' carbons, respectively, of an internal nucleotide. The nature of the reaction products is investigated, and optimal reaction conditions are reported for the construction of branched oligonucleotides. We discuss the utility of these branched DNA nanostructures for gene detection.

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Year:  2004        PMID： 14704359      PMCID： PMC373312          DOI： 10.1093/nar/gnh011

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  25 in total

1.  Enhanced detection and distinction of RNA by enzymatic probe ligation.

Authors:  M Nilsson; G Barbany; D O Antson; K Gertow; U Landegren
Journal:  Nat Biotechnol       Date:  2000-07       Impact factor: 54.908

2.  Padlock probes: circularizing oligonucleotides for localized DNA detection.

Authors:  M Nilsson; H Malmgren; M Samiotaki; M Kwiatkowski; B P Chowdhary; U Landegren
Journal:  Science       Date:  1994-09-30       Impact factor: 47.728

3.  Reverse transcriptase reads through a 2'-5'linkage and a 2'-thiophosphate in a template.

Authors:  J R Lorsch; D P Bartel; J W Szostak
Journal:  Nucleic Acids Res       Date:  1995-08-11       Impact factor: 16.971

4.  Mutation detection and single-molecule counting using isothermal rolling-circle amplification.

Authors:  P M Lizardi; X Huang; Z Zhu; P Bray-Ward; D C Thomas; D C Ward
Journal:  Nat Genet       Date:  1998-07       Impact factor: 38.330

5.  Regiospecific solid-phase synthesis of branched oligonucleotides. Effect of vicinal 2',5'- (or 2',3'-) and 3',5'-phosphodiester linkages on the formation of hairpin DNA.

Authors:  R S Braich; M J Damha
Journal:  Bioconjug Chem       Date:  1997 May-Jun       Impact factor: 4.774

6.  Enzymatic breakage and joining of deoxyribonucleic acid. 8. Hybrids of ribo- and deoxyribonucleotide homopolymers as substrates for polynucleotide ligase of bacteriophage T4.

Authors:  G C Fareed; E M Wilt; C C Richardson
Journal:  J Biol Chem       Date:  1971-02-25       Impact factor: 5.157

7.  Branched DNA signal amplification.

Authors:  M S Urdea
Journal:  Biotechnology (N Y)       Date:  1994-09

8.  Functional characterization of the T4 DNA ligase: a new insight into the mechanism of action.

Authors:  R Rossi; A Montecucco; G Ciarrocchi; G Biamonti
Journal:  Nucleic Acids Res       Date:  1997-06-01       Impact factor: 16.971

9.  Solid-phase synthesis of branched oligoribonucleotides related to messenger RNA splicing intermediates.

Authors:  M J Damha; K Ganeshan; R H Hudson; S V Zabarylo
Journal:  Nucleic Acids Res       Date:  1992-12-25       Impact factor: 16.971

10.  Specificity and fidelity of strand joining by Chlorella virus DNA ligase.

Authors:  V Sriskanda; S Shuman
Journal:  Nucleic Acids Res       Date:  1998-08-01       Impact factor: 16.971
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  6 in total

1.  Convergent and general one-step DNA-catalyzed synthesis of multiply branched DNA.

Authors:  Timothy P Mui; Scott K Silverman
Journal:  Org Lett       Date:  2008-09-23       Impact factor: 6.005

2.  Theoretical Analysis of a Self-Replicator With Reduced Template Inhibition Based on an Informational Leaving Group.

Authors:  Erwan Bigan; Henri-Philippe Mattelaer; Piet Herdewijn
Journal:  J Mol Evol       Date:  2016-03-22       Impact factor: 2.395

3.  Improved deoxyribozymes for synthesis of covalently branched DNA and RNA.

Authors:  Christine S Lee; Timothy P Mui; Scott K Silverman
Journal:  Nucleic Acids Res       Date:  2010-08-25       Impact factor: 16.971

4.  Arm-specific cleavage and mutation during reverse transcription of 2΄,5΄-branched RNA by Moloney murine leukemia virus reverse transcriptase.

Authors:  Jessica Döring; Thomas Hurek
Journal:  Nucleic Acids Res       Date:  2017-04-20       Impact factor: 16.971

5.  Dual coding potential of a 2',5'-branched ribonucleotide in DNA.

Authors:  Jessica Döring; Thomas Hurek
Journal:  RNA       Date:  2018-10-25       Impact factor: 4.942

6.  DNA ligase-mediated translation of DNA into densely functionalized nucleic acid polymers.

Authors:  Ryan Hili; Jia Niu; David R Liu
Journal:  J Am Chem Soc       Date:  2012-12-26       Impact factor: 15.419
  6 in total

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